Friedman H V, Bresler T, Garner C C, Ziv N E
Rappaport Institute and Department of Anatomy and Cell Biology, Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel.
Neuron. 2000 Jul;27(1):57-69. doi: 10.1016/s0896-6273(00)00009-x.
Time-lapse microscopy, retrospective immunohistochemistry, and cultured hippocampal neurons were used to determine the time frame of individual glutamatergic synapse assembly and the temporal order in which specific molecules accumulate at new synaptic junctions. New presynaptic boutons capable of activity-evoked vesicle recycling were observed to form within 30 min of initial axodendritic contact. Clusters of the presynaptic active zone protein Bassoon were present in all new boutons. Conversely, clusters of the postsynaptic molecule SAP90/PSD-95 and glutamate receptors were found on average only approximately 45 min after such boutons were first detected. AMPA- and NMDA-type glutamate receptors displayed similar clustering kinetics. These findings suggest that glutamatergic synapse assembly can occur within 1-2 hr after initial contact and that presynaptic differentiation may precede postsynaptic differentiation.
利用延时显微镜、回顾性免疫组织化学和培养的海马神经元来确定单个谷氨酸能突触组装的时间框架以及特定分子在新突触连接处积累的时间顺序。观察到能够进行活动诱发囊泡循环的新突触前终扣在轴突与树突初次接触后30分钟内形成。所有新终扣中都存在突触前活性区蛋白巴松管的簇集。相反，突触后分子SAP90/PSD - 95和谷氨酸受体的簇集平均在首次检测到此类终扣后约45分钟才出现。AMPA型和NMDA型谷氨酸受体表现出相似的簇集动力学。这些发现表明，谷氨酸能突触组装可在初次接触后1 - 2小时内发生，且突触前分化可能先于突触后分化。
