O'Keeffe Meredith, Hochrein Hubertus, Vremec David, Caminschi Irina, Miller Joanna L, Anders E Margot, Wu Li, Lahoud Mireille H, Henri Sandrine, Scott Bernadette, Hertzog Paul, Tatarczuch Lilliana, Shortman Ken
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia.
J Exp Med. 2002 Nov 18;196(10):1307-19. doi: 10.1084/jem.20021031.
The CD45RA(hi)CD11c(int) plasmacytoid predendritic cells (p-preDCs) of mouse lymphoid organs, when stimulated in culture with CpG or influenza virus, produce large amounts of type I interferons and transform without division into CD8(+)CD205(-) DCs. P-preDCs express CIRE, the murine equivalent of DC-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN). P-preDCs are divisible by CD4 expression into two subgroups differing in turnover rate and in response to Staphylococcus aureus. The kinetics of bromodeoxyuridine labeling and the results of transfer to normal recipient mice indicate that CD4(-) p-preDCs are the immediate precursors of CD4(+) p-preDCs. Similar experiments indicate that p-preDCs are normally long lived and are not the precursors of the short-lived steady-state conventional DCs. However, in line with the culture studies on transfer to influenza virus-stimulated mice the p-preDCs transform into CD8(+)CD205(-) DCs, distinct from conventional CD8(+)CD205(+) DCs. Hence as well as activating preexistant DCs, microbial infection induces a wave of production of a new DC subtype. The functional implications of this shift in the DC network remain to be determined.
小鼠淋巴器官中的CD45RA(hi)CD11c(int)浆细胞样前树突状细胞（p-preDCs），在用CpG或流感病毒在培养中刺激时，会产生大量I型干扰素，并在不分裂的情况下转变为CD8(+)CD205(-)树突状细胞。P-preDCs表达CIRE，它相当于小鼠的DC特异性细胞间粘附分子3结合非整合素（DC-SIGN）。P-preDCs可根据CD4表达分为两个亚组，这两个亚组在更新率和对金黄色葡萄球菌的反应方面有所不同。溴脱氧尿苷标记的动力学以及转移到正常受体小鼠的结果表明，CD4(-) p-preDCs是CD4(+) p-preDCs的直接前体。类似的实验表明，p-preDCs通常寿命较长，不是短寿命稳态常规树突状细胞的前体。然而，与转移到流感病毒刺激小鼠的培养研究一致，p-preDCs会转变为CD8(+)CD205(-)树突状细胞，与常规的CD8(+)CD205(+)树突状细胞不同。因此，除了激活已有的树突状细胞外，微生物感染还会引发一波新的树突状细胞亚型的产生。树突状细胞网络中这种转变的功能意义仍有待确定。
