逆转录病毒特异性地将氨酰-tRNA合成酶与同源引物tRNA进行包装。
Retrovirus-specific packaging of aminoacyl-tRNA synthetases with cognate primer tRNAs.
作者信息
Cen Shan, Javanbakht Hassan, Kim Sunghoon, Shiba Kiyotaka, Craven Rebecca, Rein Alan, Ewalt Karla, Schimmel Paul, Musier-Forsyth Karin, Kleiman Lawrence
机构信息
Lady Davis Institute for Medical Research and McGill AIDS Center, Jewish General Hospital, McGill University, 3755 Cote Ste-Catherine Road, Montreal, Quebec, Canada H3T 1E2.
出版信息
J Virol. 2002 Dec;76(24):13111-5. doi: 10.1128/jvi.76.24.13111-13115.2002.
The tRNAs used to prime reverse transcription in human immunodeficiency virus type 1 (HIV-1), Rous sarcoma virus (RSV), and Moloney murine leukemia virus (Mo-MuLV) are, tRNA(Trp), and tRNA(Pro), respectively. Using antibodies to the three cognate human aminoacyl-tRNA synthetases, we found that only lysyl-tRNA synthetase (LysRS) is present in HIV-1, only tryptophanyl-tRNA synthetase (TrpRS) is present in RSV, and neither these two synthetases nor prolyl-tRNA synthetase (ProRS) is present in Mo-MuLV. LysRS and TrpRS are present in HIV-1 and RSV at approximately 25 and 12 molecules/virion, respectively. These results support the hypothesis that, in HIV-1 and RSV, the cognate aminoacyl-tRNA synthetase may be used as the signal for targeting the selective packaging of primer tRNAs into retroviruses. The absence of ProRS in Mo-MuLV is consistent with reports that selective packaging of tRNA(Pro) in this virus is less important for achieving optimum annealing of the primer to Mo-MuLV genomic RNA.
用于在1型人类免疫缺陷病毒（HIV-1）、劳氏肉瘤病毒（RSV）和莫洛尼鼠白血病病毒（Mo-MuLV）中引发逆转录的tRNA分别是tRNA(Trp)和tRNA(Pro)。使用针对三种同源人类氨酰-tRNA合成酶的抗体，我们发现HIV-1中仅存在赖氨酰-tRNA合成酶（LysRS），RSV中仅存在色氨酰-tRNA合成酶（TrpRS），而Mo-MuLV中既不存在这两种合成酶，也不存在脯氨酰-tRNA合成酶（ProRS）。LysRS和TrpRS在HIV-1和RSV中的含量分别约为每病毒体25个分子和12个分子。这些结果支持了以下假设：在HIV-1和RSV中，同源氨酰-tRNA合成酶可能用作将引物tRNA选择性包装到逆转录病毒中的靶向信号。Mo-MuLV中不存在ProRS与以下报道一致，即在该病毒中tRNA(Pro)的选择性包装对于实现引物与Mo-MuLV基因组RNA的最佳退火不太重要。
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