Almeida-Toledo L F, Ozouf-Costaz C, Foresti F, Bonillo C, Porto-Foresti F, Daniel-Silva M F Z
Departamento de Biologia, Instituto de Biociências, USP, São Paulo, Brazil.
Cytogenet Genome Res. 2002;97(3-4):229-33. doi: 10.1159/000066609.
Major and 5S ribosomal genes have been localized in chromosomes from five fish species, genus Astyanax, using in situ hybridization (FISH) with 28S and 5S rDNA probes. In situ signals for the major rDNA co-localized with the 5S rDNA clusters in the pericentromeric region of one marker chromosome in all five species analyzed. The conserved localization of these two rDNA clusters in the five related Astyanax species was considered as indicative of a close relationship among them. The use of these molecular markers for elucidating evolutionary relationships among closely related taxa is discussed.
利用28S和5S rDNA探针的荧光原位杂交（FISH）技术，已将主要核糖体基因和5S核糖体基因定位到了丽脂鲤属五种鱼类的染色体上。在所分析的所有五个物种中，主要rDNA的原位信号与5S rDNA簇共定位于一条标记染色体的着丝粒周围区域。这两个rDNA簇在五个相关丽脂鲤物种中的保守定位被认为表明它们之间存在密切关系。本文讨论了利用这些分子标记来阐明近缘分类群之间的进化关系。
