猪源S-腺苷同型半胱氨酸水解酶AHCY基因的鉴定与染色体定位

Characterization and chromosome assignment of the porcine AHCY gene for S-adenosylhomocysteine hydrolase.

作者信息

Leeb T, Rohrer G A

机构信息

Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Hannover, Germany.

出版信息

Cytogenet Genome Res. 2002;97(1-2):116-9. doi: 10.1159/000064066.

DOI:10.1159/000064066

PMID:12438749

Abstract

The gene for S-adenosylhomocysteine hydrolase (AHCY) is involved in the regulation of cellular methylation reactions. Here we report the cloning, sequencing, and chromosomal assignment of the porcine AHCY gene. The gene consists of 10 exons spanning approximately 19 kb of genomic DNA. It encodes a protein of 432 amino acids that shows about 96% identity to the orthologous S-adenosylhomocysteine hydrolases from human, rat, and mouse. The porcine AHCY gene is located very close to the agouti signaling protein gene (ASIP) on SSC17q21. The chromosomal localization was subsequently confirmed by RH mapping and the genetic mapping of an intragenic microsatellite that maps to 57 cM on the linkage map of SSC17.

摘要

S-腺苷同型半胱氨酸水解酶（AHCY）基因参与细胞甲基化反应的调控。在此，我们报告猪AHCY基因的克隆、测序及染色体定位。该基因由10个外显子组成，跨越约19 kb的基因组DNA。它编码一种含432个氨基酸的蛋白质，与来自人、大鼠和小鼠的直系同源S-腺苷同型半胱氨酸水解酶具有约96%的同一性。猪AHCY基因位于猪17号染色体q21区域，非常靠近刺鼠信号蛋白基因（ASIP）。随后通过辐射杂种图谱和一个基因内微卫星的遗传图谱对染色体定位进行了确认，该微卫星在猪17号染色体连锁图谱上定位于57 cM处。