Bacon Andrea, Holmes Fiona E, Small Caroline J, Ghatei Mohammed, Mahoney Sally, Bloom Stephen, Wynick David
University Research Centre for Neuroendocrinology, Bristol University, Marlborough Street, Bristol BS2 8HW, UK.
Neuroreport. 2002 Nov 15;13(16):2129-32. doi: 10.1097/00001756-200211150-00028.
The 29 amino acid neuropeptide galanin is normally expressed in < 5% of sensory neurons in the adult dorsal root ganglia. After nerve transection (axotomy), the galanin content of the dorsal root ganglia rises 120-fold and the peptide is then expressed in > 50% of neurons. Published data suggest that galanin plays a role in the modulation of pain processing and may be involved in the regeneration of sensory neurons. Here we describe the initial characterisation of a new line of transgenic mice that selectively over-express galanin in the dorsal root ganglia in an inducible manner following axotomy of the sciatic nerve, but not in the uninjured state. Results of acute thermal or mechanosensory pain tests are normal in intact transgenic animals when compared to wild-type controls. The generation of these novel transgenic animals will be most useful as genetic tools to further elucidate the role played by galanin in the adaptive response of the peripheral nervous system to injury.
29个氨基酸的神经肽甘丙肽通常在成年背根神经节中不到5%的感觉神经元中表达。神经切断（轴突切断）后，背根神经节中的甘丙肽含量增加120倍，然后该肽在超过50%的神经元中表达。已发表的数据表明，甘丙肽在疼痛处理的调节中起作用，并且可能参与感觉神经元的再生。在这里，我们描述了一种新的转基因小鼠品系的初步特征，该品系在坐骨神经轴突切断后以可诱导的方式在背根神经节中选择性地过度表达甘丙肽，但在未受伤状态下不表达。与野生型对照相比，完整的转基因动物的急性热或机械感觉疼痛测试结果正常。这些新型转基因动物的产生将作为遗传工具非常有用，以进一步阐明甘丙肽在周围神经系统对损伤的适应性反应中所起的作用。
