Papon Jean-François, Coste André, Gendron Marie-Claude, Cordonnier Catherine, Wingerstmann Laure, Peynègre Roger, Escudier Estelle
Institut National de la Santé et de la Recherche Médicale, Unité U492, Faculté de Médecine, Paris XII, Créteil, France.
Laryngoscope. 2002 Nov;112(11):2067-75. doi: 10.1097/00005537-200211000-00030.
OBJECTIVE/HYPOTHESIS: Through human leukocyte antigen-DR (HLA-DR) and intercellular adhesion molecule-1 (ICAM-1) expression, nasal epithelial cells could actively participate in the chronic inflammation and eosinophil infiltration observed in nasal polyps. The objective of the study was to evaluate HLA-DR and ICAM-1 expression in polyp epithelium and in a culture model of polyp epithelial cells allowing ciliated and secretory differentiation.
Prospective non-randomized controlled in vitro study.
The in vitro HLA-DR and ICAM-1 expression was studied under basal conditions or after exposure to interferon-gamma, transforming growth factor-beta1, lipopolysaccharide, dexamethasone, or cetirizine. HLA-DR and ICAM-1 expression was investigated in situ by immunohistochemical staining of polyps and in vitro by immunofluorescent staining of cell cultures. HLA-DR and ICAM-1 were localized in cultured cells by confocal microscopy. Cultured cells expressing HLA-DR and ICAM-1 were quantified by flow cytometry.
Both HLA-DR and ICAM-1 showed significant immunostaining of nasal polyp epithelium. In nasal polyp epithelial cell cultures, less than 5% of cells were positive for HLA-DR whereas 40% were positive for ICAM-1 at day 3. In vitro, HLA-DR was mainly located in the cytoplasm and ICAM-1 predominated on the apicolateral cytoplasmic membrane. Comparison of in situ and in vitro results showed that well-differentiated and poorly differentiated cells predominantly expressed HLA-DR and ICAM-1, respectively. Interferon-gamma significantly increased HLA-DR and ICAM-1 expression, whereas transforming growth factor-beta1 significantly decreased HLA-DR expression and lipopolysaccharide significantly increased ICAM-1 expression.
HLA-DR and ICAM-1 epithelial expression in nasal polyps in situ and in vitro and their in vitro modulation reinforce the active role of epithelial cells in chronic inflammatory diseases of the upper airways.
目的/假设：通过人类白细胞抗原-DR（HLA-DR）和细胞间黏附分子-1（ICAM-1）的表达，鼻上皮细胞可积极参与鼻息肉中观察到的慢性炎症和嗜酸性粒细胞浸润。本研究的目的是评估息肉上皮以及允许纤毛和分泌分化的息肉上皮细胞培养模型中HLA-DR和ICAM-1的表达。
前瞻性非随机对照体外研究。
在基础条件下或暴露于干扰素-γ、转化生长因子-β1、脂多糖、地塞米松或西替利嗪后，研究体外HLA-DR和ICAM-1的表达。通过息肉的免疫组织化学染色在原位研究HLA-DR和ICAM-1的表达，并通过细胞培养物的免疫荧光染色在体外进行研究。通过共聚焦显微镜将HLA-DR和ICAM-1定位在培养细胞中。通过流式细胞术对表达HLA-DR和ICAM-1的培养细胞进行定量。
HLA-DR和ICAM-1在鼻息肉上皮中均显示出明显的免疫染色。在鼻息肉上皮细胞培养物中，第3天时，不到5%的细胞HLA-DR呈阳性，而40%的细胞ICAM-1呈阳性。在体外，HLA-DR主要位于细胞质中，ICAM-1主要位于顶端外侧细胞质膜上。原位和体外结果的比较表明，分化良好和分化不良的细胞分别主要表达HLA-DR和ICAM-1。干扰素-γ显著增加HLA-DR和ICAM-1的表达，而转化生长因子-β1显著降低HLA-DR的表达，脂多糖显著增加ICAM-1的表达。
鼻息肉原位和体外上皮中HLA-DR和ICAM-1的表达及其体外调节加强了上皮细胞在上呼吸道慢性炎症疾病中的积极作用。
