Liu Tian-yi, Dong Zhen
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan, Union Hospital, Jilin University, Changchun 130031, China.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 2003 Jun;38(3):183-6.
To explore the effect of proinflammatory cytokines on the expression of human leucocyte antigen(HLA-DR) in epithelial cell of nasal polyps.
The samples were from 23 patients with nasal polyps and 11 cases of normal inferior turbinates. The samples were fixed, paraffin-embeded and sectioned for observing distribution of HLA-DR positive cells. Nasal epithelial cells were obtained after enzymatic digestion and cell culture was performed for observing influences of interleukin (IL)-1 beta and tumor necrosis factor (TNF-alpha) to expression of HLA-DR of epithelial cells and the effect of dexamethasone(10(-5) mol/L).
The HLA-DR expression in epithelium was significantly stronger in nasal polyps than that in inferior turbinates. The expression of HLA-DR in cultured cells under stimulation of proinflammatory cytokines was increased compared with controls, but the expression was more intense in nasal polyps than that in inferior turbinates. Increased HLA-DR expression under stimulation of cytokines was reduced significantly by dexamethasone.
The epithelium is in a key position to participate in inflammatory and immunologic events in the airways. Epithelial cells potentially contribute to airway inflammation by antigen presentation and the production of proinflammatory cytokines.
探讨促炎细胞因子对鼻息肉上皮细胞中人白细胞抗原（HLA-DR）表达的影响。
样本来自23例鼻息肉患者和11例正常下鼻甲。样本经固定、石蜡包埋和切片，以观察HLA-DR阳性细胞的分布。酶消化后获取鼻上皮细胞并进行细胞培养，以观察白细胞介素（IL）-1β和肿瘤坏死因子（TNF-α）对上皮细胞HLA-DR表达的影响以及地塞米松（10⁻⁵mol/L）的作用。
鼻息肉上皮中HLA-DR的表达明显强于下鼻甲。与对照组相比，促炎细胞因子刺激下培养细胞中HLA-DR的表达增加，但鼻息肉中的表达比下鼻甲更强烈。地塞米松可显著降低细胞因子刺激下HLA-DR表达的增加。
上皮在气道炎症和免疫反应中起关键作用。上皮细胞可能通过抗原呈递和促炎细胞因子的产生促进气道炎症。
