Bard E, Laibe S, Clair S, Biichlé S, Millon L, Drobacheff C, Bettinger D, Seillès E, Meillet D
Institut d'Etude et de Transfert de Gènes EA3181, Faculté de Médicine-Pharmacie, Besançon, France.
J Acquir Immune Defic Syndr. 2002 Nov 1;31(3):276-84. doi: 10.1097/00126334-200211010-00002.
Buccal and digestive tract opportunistic infections occur frequently in patients infected by HIV. In this study, we measured lysozyme (Lz), lactoferrin (Lf), total IgA (T-IgA), and secretory IgA (S-IgA) levels to investigate nonspecific secretory immunity in HIV-infected patients with oral candidiasis. Serum, saliva, and stool samples were analyzed by time-resolved immunofluorometric assay for Lz and Lf levels and by enzyme-linked immunosorbent assay for T-IgA and S-IgA levels. Mean salivary Lf and T-IgA levels (66.50 mg/L and 0.10 g/L, respectively) and mean fecal Lf, T-IgA, and S-IgA outputs (0.87, 54.0, and 43.6 mg/d, respectively) were significantly higher in HIV-infected patients with oropharyngeal candidiasis than in HIV-infected patients without oropharyngeal candidiasis and healthy subjects. There was a modification in the molecular form rate, with a high increase in S-IgA and monomeric IgA transudation from the plasmatic compartment into salivary and digestive fluids and an increase in salivary Lf local synthesis by polymorphonuclear neutrophils. HIV infection appears to be associated with dysregulation of some of the nonspecific immune factors at the mucosal surface. Despite high saliva concentrations and high intestinal output, innate immunity was not able to stop yeast expansion in HIV-infected patients.
口腔和消化道机会性感染在感染HIV的患者中频繁发生。在本研究中，我们测量了溶菌酶（Lz）、乳铁蛋白（Lf）、总IgA（T-IgA）和分泌型IgA（S-IgA）水平，以研究患有口腔念珠菌病的HIV感染患者的非特异性分泌免疫。通过时间分辨免疫荧光分析法分析血清、唾液和粪便样本中的Lz和Lf水平，通过酶联免疫吸附测定法分析T-IgA和S-IgA水平。患有口咽念珠菌病的HIV感染患者的唾液Lf和T-IgA平均水平（分别为66.50 mg/L和0.10 g/L）以及粪便Lf、T-IgA和S-IgA平均排出量（分别为0.87、54.0和43.6 mg/d）显著高于未患有口咽念珠菌病的HIV感染患者和健康受试者。分子形式率发生了改变，S-IgA和单体IgA从血浆区室向唾液和消化液的渗出大幅增加，多形核中性粒细胞对唾液Lf的局部合成增加。HIV感染似乎与黏膜表面一些非特异性免疫因子的失调有关。尽管唾液浓度高且肠道排出量高，但固有免疫无法阻止HIV感染患者体内酵母菌的扩散。
