Smith Jeffrey B, Wadleigh David J, Xia Yu-Rong, Mar Rebecca A, Herschman Harvey R, Lusis Aldons J
Pediatrics/Neonatology B2-325, MDCC, David Geffen School of Medicine and Mattel Children's Hospital at UCLA, 10833 Le Conte Avenue, Los Angeles, CA 90095-1752, USA.
Immunogenetics. 2002 Nov;54(8):599-603. doi: 10.1007/s00251-002-0501-5. Epub 2002 Oct 1.
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). To clarify the relationship of LIX to human ENA-78 and GCP-2, we cloned and mapped the LIX gene. The organization of the LIX gene ( Scyb5) is similar to those of the human ENA-78 ( SCYB5) and GCP-2 ( SCYB6) genes. The intron-exon boundaries of the three genes are exactly conserved, and the introns have similar sizes. The first 100 bp of the 5' flanking regions are highly similar, with conserved NF-kappaB and GATA sites in identical positions in all three genes. Further 5', the Lix flanking region sequence diverges from those of ENA-78 and GCP-2, which remain highly similar for 350 bp preceding the start sites. Using a (C57BL/6 J x Mus spretus) F1 x C57BL/6J backcross panel, Lix was mapped to a locus near D5Ucla5 at 49.0 cM on Chromosome (Chr) 5. Mapping with the T31 radiation hybrid panel placed Lix between D5Mit360 and D5Mit6. Physical maps of the CXC chemokine clusters on murine Chr 5 and human Chr 21 were constructed using the Celera mouse genome database and the public human genome database. The sequence and mapping data suggest that the human ENA78-PBP-PF4 and GCP2- psi PBP-PF4V1 loci arose from an evolutionarily recent duplication of an ancestral locus related to the murine Lix-Pbp-Pf4 locus.
脂多糖诱导的CXC趋化因子（LIX）是一种小鼠趋化因子，类似于两种人类趋化因子，即ENA-78（CXCL5）和GCP-2（CXCL6）。为了阐明LIX与人类ENA-78和GCP-2的关系，我们克隆并定位了LIX基因。LIX基因（Scyb5）的结构与人类ENA-78（SCYB5）和GCP-2（SCYB6）基因相似。这三个基因的内含子-外显子边界完全保守，且内含子大小相似。5'侧翼区域的前100 bp高度相似，在所有三个基因的相同位置存在保守的核因子κB和GATA位点。在5'侧翼区域更远处，Lix侧翼区域序列与ENA-78和GCP-2的序列不同，后者在起始位点之前的350 bp内仍高度相似。使用（C57BL/6 J×小家鼠）F1×C57BL/6J回交群体，将Lix定位到位于5号染色体（Chr）上49.0 cM处的D5Ucla5附近的一个位点。利用T31辐射杂种群体进行定位，将Lix定位于D5Mit360和D5Mit6之间。利用Celera小鼠基因组数据库和公共人类基因组数据库构建了小鼠Chr 5和人类Chr 21上CXC趋化因子簇的物理图谱。序列和定位数据表明，人类ENA78-PBP-PF4和GCP2-ψPBP-PF4V1基因座源于与小鼠Lix-Pbp-Pf4基因座相关的祖先基因座在进化上最近的一次复制。
