Suto J, Sekikawa K
Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Int J Obes Relat Metab Disord. 2002 Nov;26(11):1517-9. doi: 10.1038/sj.ijo.0802152.
KK and KK-A(y) mice serve as animal models of non-insulin-dependent diabetes mellitus with moderate obesity. Quantitative trait locus (QTL) analysis was performed to identify gene loci that account for fasting hyperglycemia, glucose intolerance and plasma insulin in 91 F(2) females of a C57BL/6JxKK-A(y) intercross. For glucose intolerance, a significant QTL was identified on chromosome 8, with a maximum lod score of 5.6. This locus had strong influence on the late phase of the intraperitoneal glucose tolerance test (IPGTT), suggesting that the locus may have role in glucose clearance rather than in mere hyperglycemia. In addition, three suggestive QTLs were identified on chromosomes 1 (fasting glucose), 3 (fasting insulin) and 4, (blood glucose at 120 min during IPGTT, and glucose intolerance).
KK 小鼠和 KK-A(y) 小鼠作为伴有中度肥胖的非胰岛素依赖型糖尿病动物模型。进行了数量性状基因座（QTL）分析，以确定在 C57BL/6J×KK-A(y) 杂交的 91 只 F(2) 雌性小鼠中导致空腹高血糖、葡萄糖不耐受和血浆胰岛素水平变化的基因座。对于葡萄糖不耐受，在 8 号染色体上鉴定出一个显著的 QTL，最大对数优势得分为 5.6。该基因座对腹腔内葡萄糖耐量试验（IPGTT）的后期有强烈影响，表明该基因座可能在葡萄糖清除中起作用，而不仅仅是在单纯的高血糖中起作用。此外，在 1 号染色体（空腹血糖）、3 号染色体（空腹胰岛素）和 4 号染色体（IPGTT 期间 120 分钟时的血糖以及葡萄糖不耐受）上鉴定出三个提示性 QTL。
