Horn P L, Leeman K, Pyne D B, Gore C J
University of Canberra, Division of Science and Design, ACT, Australia.
Int J Sports Med. 2002 Nov;23(8):595-9. doi: 10.1055/s-2002-35524.
This study was undertaken to determine the effects of acute intense exercise on the cell membrane-bound glycoprotein designated cluster of differentiation (CD) 94. This marker on natural killer (NK) lymphocytes contributes to control of cell function. CD94 was measured on natural killer lymphocytes from 11 adult (average 25 yrs), well-trained male subjects, (Vdot;O 2 peak mean, 5.01 L x min -1) before and immediately after a final, 4 min all-out, cycle ergometry test. Using flow cytometry, lymphocyte populations were distinguished as either having (CD94 +) or lacking (CD94 -) the cell marker. The absolute number of CD94 + and CD94 - natural killer cells increased with exercise but the mean fluorescence intensity (MFI) for CD94 decreased from pre 131, to post 117 (p = 0.01). The percentage of NK cells that were CD94 + did not change, but exercise did mobilise natural killer cells of greater MFI for the surface markers designated CD16/CD56 (pre 750 to post 1 050, p < 0.001). The latter suggests that some exercise-mobilised natural killer cells may have originated from the liver as CD56 +bright cells.
本研究旨在确定急性剧烈运动对称为分化簇（CD）94的细胞膜结合糖蛋白的影响。自然杀伤（NK）淋巴细胞上的这一标志物有助于控制细胞功能。在11名成年（平均25岁）、训练有素的男性受试者（峰值摄氧量均值为5.01L·min⁻¹）进行最后一次4分钟全力蹬车测力计测试之前和之后，对其NK淋巴细胞上的CD94进行了测量。使用流式细胞术，淋巴细胞群体被区分为具有（CD94⁺）或缺乏（CD94⁻）该细胞标志物。CD94⁺和CD94⁻自然杀伤细胞的绝对数量随运动增加，但CD94的平均荧光强度（MFI）从运动前的131降至运动后的117（p = 0.01）。CD94⁺的NK细胞百分比没有变化，但运动确实调动了表面标志物为CD16/CD56的MFI更高的自然杀伤细胞（运动前为750，运动后为1050，p < 0.001）。后者表明，一些运动调动的自然杀伤细胞可能作为CD56⁺亮细胞起源于肝脏。
