Madhavan H N, Priya K
L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, India.
Indian J Med Res. 2002 Jun;115:242-7.
BACKGROUND & OBJECTIVES: Since cytomegalovirus (CMV) is heterogenous and exhibits genomic polymorphism, polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) was applied to identify the glycoprotein B subtypes in patients diagnosed to have CMV disease.
CMV standard strain (AD 169) and 55 clinical specimens from 50 patients (35 males; 15 females) positive for CMV by PCR coding for the morphological transforming region II gene were subjected to PCR coding for gp 55 region of CMV gB gene. PCR amplified products were subjected to RFLP using Hinf I.
Of 50 patients, 26 (52.0%) (20 males; 6 females) were positive by PCR (gB gene). Upon RFLP, AD 169 and CMV strains of 14 (53.8%) patients (14 males) were typed as subtype II, and 12 (46.1%) (6 males; 6 females) as subtype III. Of 11 paediatric patients, 7 (63.6%) were infected with CMV subtype II and 4 (36.4%) with subtype III. CMV strains of the dual specimens from 3 PCR positive patients belonged to the same subtype.
INTERPRETATION & CONCLUSION: In this study on CMV genotypes, CMV gB subtypes II and III were found in 53.8 and 46.1 per cent patients studied respectively, while I and IV were not present.
由于巨细胞病毒（CMV）具有异质性且存在基因组多态性，因此应用基于聚合酶链反应（PCR）的限制性片段长度多态性（RFLP）技术来鉴定诊断为CMV疾病患者的糖蛋白B亚型。
对CMV标准株（AD 169）以及50例患者（35例男性；15例女性）的55份临床标本进行检测，这些标本经PCR检测CMV形态转化区II基因呈阳性，对其进行CMV gB基因gp 55区域的PCR扩增。PCR扩增产物用Hinf I进行RFLP分析。
50例患者中，26例（52.0%）（20例男性；6例女性）PCR（gB基因）检测呈阳性。经RFLP分析，14例患者（14例男性）（53.8%）的AD 169和CMV毒株被分型为II型，12例（46.1%）（6例男性；6例女性）为III型。11例儿科患者中，7例（63.6%）感染CMV II型，4例（36.4%）感染III型。3例PCR阳性患者的双份标本中的CMV毒株属于同一亚型。
在这项关于CMV基因型的研究中，分别在53.8%和46.1%的研究患者中发现了CMV gB II型和III型，未发现I型和IV型。
