Robertson Kindal M, Tieleman D Peter
Department of Biological Sciences, University of Calgary, AB, Canada.
Biochem Cell Biol. 2002;80(5):517-23. doi: 10.1139/o02-145.
OmpF and PhoE from Escherichia coli and related homologous proteins from other Gram-negative bacteria allow the passive transport of small polar molecules across the bacterial outer membrane. In vitro, they exhibit voltage gating depending on the experimental conditions. We review current hypotheses on the underlying molecular mechanism of voltage gating of OmpF porin and show how computer simulations can be used to examine each of the proposed mechanisms.
来自大肠杆菌的OmpF和PhoE以及其他革兰氏阴性细菌的相关同源蛋白允许小极性分子被动转运穿过细菌外膜。在体外，它们根据实验条件表现出电压门控。我们综述了关于OmpF孔蛋白电压门控潜在分子机制的当前假说，并展示了计算机模拟如何用于检验每种提出的机制。
