Fedor M J
Department of Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, MB35, 10550 N. Torrey Pines Road, La Jolla CA 92037, USA.
Biochem Soc Trans. 2002 Nov;30(Pt 6):1109-15. doi: 10.1042/bst0301109.
Evidence that hairpin ribozymes function in the absence of bivalent cation cofactors suggests that active site nucleobases might participate directly in catalytic chemistry. We have adopted an abasic ribozyme rescue strategy to begin to dissect the roles of specific nucleobases in hairpin ribozyme activity. Loss of one active site nucleobase, G8, could be compensated by providing certain nucleobases and nucleobase analogues in solution. Comparison of the biochemical and structural features that are shared among small molecules that mediate rescue provides a new perspective on potential mechanisms of hairpin ribozyme catalysis.
发夹状核酶在没有二价阳离子辅助因子的情况下仍能发挥作用，这一证据表明活性位点的核碱基可能直接参与催化化学反应。我们采用了一种无碱基核酶拯救策略，以开始剖析特定核碱基在发夹状核酶活性中的作用。通过在溶液中提供某些核碱基和核碱基类似物，可以补偿一个活性位点核碱基G8的缺失。对介导拯救的小分子之间共有的生化和结构特征进行比较，为发夹状核酶催化的潜在机制提供了新的视角。
