Yamada H, Yoshida H, Nakazawa H, Kumagai H
Acta Vitaminol Enzymol. 1975;29(1-6):248-51.
Crystalline tryptophanase from Proteus rettgeri was shown to catalyze the synthesis of L-tryptophan from pyruvate, ammonia and indole, at maximum velocity approaching that of the degradative reaction. Based on the results obtained with the crystalline tryptophanase, an enzymatic method for preparation of L-tryptophan and its related amino acids was developed. The cells of Proteus rettgeri containing high enzymatic activity were used directly as the enzyme. This method is simple and is one of the most economical processes to date for preparing L-tryptophan related amino acids from starting materials: sodium pyruvate, indole and its derivatives.
变形杆菌的结晶色氨酸酶被证明能催化由丙酮酸、氨和吲哚合成L-色氨酸，其最大反应速度接近降解反应的速度。基于用结晶色氨酸酶获得的结果，开发了一种制备L-色氨酸及其相关氨基酸的酶法。含有高酶活性的变形杆菌细胞直接用作酶。该方法简单，是迄今为止从丙酮酸、吲哚及其衍生物等起始原料制备L-色氨酸相关氨基酸最经济的方法之一。
