Lenartovicz Veridiana, Marques de Souza Cristina Giatti, Moreira Fabiana Guillen, Peralta Rosane Marina
Departamento de Bioquimica, Universidade Estadual de Maringá, 87.020-900, Maringá, PR, Brazil.
J Basic Microbiol. 2002;42(6):388-95. doi: 10.1002/1521-4028(200212)42:6<388::AID-JOBM388>3.0.CO;2-H.
This work has evaluated the temperature effect in the production of multiple xylanases by a locally isolated strain of Aspergillus fumigatus Fresenius. Three isoenzymes, identified as xylanases I, II, and III with apparent molecular weight of 45.7 KDa, 39.8 KDa and 18.2 KDa, respectively, were produced in cultures developed at 30 degrees C and at 42 degrees C. The pattern of distribution of xylanase activity among the three isoenzymes was greatly affected by the growth temperature: at 30 degrees C, the total xylanase activity was distributed homogeneously among the three enzymes, while at 42 degrees C, the total xylanase activity was mainly due to the fractions with the highest MW (I and II) and the xylanase III was a minor component.
本研究评估了本地分离的烟曲霉弗里斯菌株生产多种木聚糖酶时的温度效应。在30℃和42℃培养的菌株中产生了三种同工酶，分别鉴定为木聚糖酶I、II和III，其表观分子量分别为45.7 kDa、39.8 kDa和18.2 kDa。三种同工酶之间木聚糖酶活性的分布模式受生长温度的影响很大：在30℃时，总木聚糖酶活性均匀分布于这三种酶中，而在42℃时，总木聚糖酶活性主要归因于分子量最高的组分（I和II），木聚糖酶III是次要成分。
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