Garai Debabrata, Kumar Vineet
Biochemical Engineering Laboratory, Department of Chemical Engineering, Indian Institute of Technology, Roorkee, Uttarakhand, India.
3 Biotech. 2013 Apr;3(2):127-136. doi: 10.1007/s13205-012-0077-1. Epub 2012 Jul 26.
In this study, a novel isolate Aspergillus candidus was employed for xylanase production using low cost agro residues. A Box-Behnken design matrix was used to optimize the influential parameters like carbon source, nitrogen source and incubation temperature for maximum xylanase production. Under optimized condition, enzyme titer level enhanced to 69 IU/ml at 48 h with volumetric productivity 1437 IU/l h. Growth and enzyme production were observed even at pH 11.0, indicating its ability to sustain at high alkaline environment. Little amount of cellulase was produced concomitantly with xylanase during the course of the process. Volumetric productivity of xylanase was found as a function of temperature. This fungal strain was emerged as a one among few strains having high xylanase productivity.
在本研究中，使用新型分离菌株白色念珠菌曲霉，以低成本农业残留物为原料生产木聚糖酶。采用Box-Behnken设计矩阵优化影响木聚糖酶产量的参数，如碳源、氮源和培养温度，以实现木聚糖酶的最大产量。在优化条件下，48小时时酶活力水平提高到69 IU/ml，体积产率为1437 IU/l·h。即使在pH 11.0时也能观察到生长和酶的产生，表明其在高碱性环境中维持生长的能力。在该过程中，伴随着木聚糖酶的产生，同时产生了少量纤维素酶。发现木聚糖酶的体积产率是温度的函数。该真菌菌株是少数具有高木聚糖酶生产率的菌株之一。
