Lee Yeonhee, Lee Choong-Sik, Kim Yeo-Jung, Chun Sungmin, Park Seohyung, Kim Young Sun, Han Byoung-Don
Department of Biotechnology, Seoul Women's University, Korea.
Mol Cells. 2002 Oct 31;14(2):192-7.
A robust and fast DNA chip method was developed in order to detect the various beta-lactam antibiotic-resistance genes in one slide. These genes included PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY, and AmpC. beta-lactam antibiotic-resistance genes were labeled with a fluorescent nucleotide by a multiplex polymerase chain reaction using a mixture of specific primer sets for each gene. This labeled target was hybridized with a DNA chip that contained the spots of the specific probe DNAs for each beta-lactam antibiotic-resistance gene. This technique made it possible to detect the specific resistance gene, even in a single bacterium.
为了在一张载玻片上检测各种β-内酰胺类抗生素抗性基因，开发了一种强大且快速的DNA芯片方法。这些基因包括PSE、OXA、FOX、MEN、CMY、TEM、SHV、OXY和AmpC。通过使用针对每个基因的特异性引物组混合物进行多重聚合酶链反应，用荧光核苷酸标记β-内酰胺类抗生素抗性基因。这种标记的靶标与包含每个β-内酰胺类抗生素抗性基因特异性探针DNA斑点的DNA芯片杂交。即使在单个细菌中，该技术也能够检测到特定的抗性基因。
