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Rapid detection of methicillin-resistant Staphylococcus aureus directly from sterile or nonsterile clinical samples by a new molecular assay.通过一种新的分子检测方法直接从无菌或非无菌临床样本中快速检测耐甲氧西林金黄色葡萄球菌。
J Clin Microbiol. 2003 Jan;41(1):254-60. doi: 10.1128/JCM.41.1.254-260.2003.
2
Linezolid resistance in clinical isolates of Staphylococcus aureus.金黄色葡萄球菌临床分离株中的利奈唑胺耐药性。
J Antimicrob Chemother. 2003 Jan;51(1):186-8. doi: 10.1093/jac/dkg104.
3
Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis.耐甲氧西林金黄色葡萄球菌与甲氧西林敏感金黄色葡萄球菌菌血症相关死亡率的比较：一项荟萃分析。
Clin Infect Dis. 2003 Jan 1;36(1):53-9. doi: 10.1086/345476. Epub 2002 Dec 13.
4
Development of DNA chip for the simultaneous detection of various beta-lactam antibiotic-resistant genes.用于同时检测多种β-内酰胺类抗生素耐药基因的DNA芯片的研制
Mol Cells. 2002 Oct 31;14(2):192-7.
5
Rapid detection of methicillin-resistant staphylococci from blood culture bottles by using a multiplex PCR assay.使用多重聚合酶链反应分析法从血培养瓶中快速检测耐甲氧西林葡萄球菌
J Clin Microbiol. 2002 Aug;40(8):2786-90. doi: 10.1128/JCM.40.8.2786-2790.2002.
6
Staphylococcus aureus resistant to vancomycin--United States, 2002.2002年美国出现对万古霉素耐药的金黄色葡萄球菌
MMWR Morb Mortal Wkly Rep. 2002 Jul 5;51(26):565-7.
7
The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA).耐甲氧西林金黄色葡萄球菌（MRSA）的进化史。
Proc Natl Acad Sci U S A. 2002 May 28;99(11):7687-92. doi: 10.1073/pnas.122108599.
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Changing pattern of antibiotic resistance in methicillin-resistant Staphylococcus aureus from German hospitals.德国医院耐甲氧西林金黄色葡萄球菌抗生素耐药性的变化模式
Infect Control Hosp Epidemiol. 2001 Nov;22(11):683-6. doi: 10.1086/501845.
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Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci.基于转录谱分析鉴定受agr和/或sarA基因座调控的金黄色葡萄球菌基因。
J Bacteriol. 2001 Dec;183(24):7341-53. doi: 10.1128/JB.183.24.7341-7353.2001.
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Methicillin-resistant, quinupristin-dalfopristin-resistant Staphylococcus aureus with reduced sensitivity to glycopeptides.对甲氧西林耐药、对奎奴普丁-达福普汀耐药且对糖肽类敏感性降低的金黄色葡萄球菌。
J Clin Microbiol. 2001 Oct;39(10):3586-90. doi: 10.1128/JCM.39.10.3586-3590.2001.

用于同时检测金黄色葡萄球菌中九个临床相关抗生素抗性基因的多重聚合酶链反应检测法。

Multiplex PCR assay for simultaneous detection of nine clinically relevant antibiotic resistance genes in Staphylococcus aureus.

作者信息

Strommenger Birgit, Kettlitz Christiane, Werner Guido, Witte Wolfgang

机构信息

Robert Koch Institute, Wernigerode Branch, D-38855 Wernigerode, Germany.

出版信息

J Clin Microbiol. 2003 Sep;41(9):4089-94. doi: 10.1128/JCM.41.9.4089-4094.2003.

DOI:10.1128/JCM.41.9.4089-4094.2003
PMID:12958230
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC193808/
Abstract

In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK, tetM (tetracycline resistance), erm(A), erm(C) (macrolide-lincosamide-streptogramin B resistance), vat(A), vat(B), and vat(C) (streptogramin A resistance) simultaneously in one PCR amplification. An additional primer pair for the amplification of a fragment of the staphylococcal 16S rDNA was included as a positive control. The multiplex PCR assay was evaluated on 30 different S. aureus isolates, and the PCR results correlated with the phenotypic antibiotic resistance data obtained by the broth microdilution assay. The multiplex PCR assay offers a rapid, simple, and accurate identification of antibiotic resistance profiles and could be used in clinical diagnosis as well as for the surveillance of the spread of antibiotic resistance determinants in epidemiological studies.

摘要

在本研究中，我们描述了一种用于检测金黄色葡萄球菌9种临床相关抗生素抗性基因的多重PCR检测方法。对条件进行了优化，以便在一次PCR扩增中同时扩增mecA（编码耐甲氧西林）、aacA-aphD（氨基糖苷抗性）、tetK、tetM（四环素抗性）、erm(A)、erm(C)（大环内酯-林可酰胺-链阳菌素B抗性）、vat(A)、vat(B)和vat(C)（链阳菌素A抗性）的片段。另外包含一对用于扩增葡萄球菌16S rDNA片段的引物作为阳性对照。对30种不同的金黄色葡萄球菌分离株进行了多重PCR检测评估，PCR结果与通过肉汤微量稀释法获得的表型抗生素抗性数据相关。该多重PCR检测方法可快速、简单且准确地鉴定抗生素抗性谱，可用于临床诊断以及在流行病学研究中监测抗生素抗性决定因素的传播。