DL-聚乳酸/羟基磷灰石/脱钙骨基质骨修复复合材料的制备及降解特性研究

Preparation and degradation characteristic study of bone repair composite of DL-polylactic acid/hydroxyapatite/decalcifying bone matrix.

作者信息

Zhao Jianhua, Liao Weihong, Wang Yuanliang, Pan Jun, Liu Feng

机构信息

Department of Orthopaedics, Daping Hospital/Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.

出版信息

Chin J Traumatol. 2002 Dec;5(6):369-73.

PMID:12443580

Abstract

OBJECTIVE

To explore the preparative method and study the degradation characteristics of bone repair composite of DL-polylactic acid (PDLLA)/hydroxyapatite(HA)/decalcifying bone matrix (DBM) in vitro.

METHODS

An emulsion blend method was developed to prepare the composite of PDLLA/HA/DBM in weight ratio of PDLLA:HA:DBM = 1.5-2:1-1.5:1. The dynamic changes of weight, biomechanical property and pH value of PDLLA/HA/DBM and PDLLA in phosphate buffered saline (PBS, pH 7.4) were studied respectively through degradation tests in vitro.

RESULTS

Without being heated, PDLLA, HA and DBM could be synthesized with the emulsion blend method as bone composite of PDLLA/HA/DBM, which had both osteoconductive and osteoinductive effects. The diameter of the aperture was 100-400 microm and the gap rate was 71.3%. During degradation, the pH value of PDLLA solution decreased lightly within 2 weeks, but decreased obviously at the end of 4 weeks and the value was 4.0. While the pH value of PDLLA/HA/DBM kept quite steady and was 6.4 at the end of 12 weeks. The weight of PDLLA changed little within 4 weeks, then changed obviously and was 50% of its initial weight at the end of 12 weeks. While the weight of PDLLA/HA/DBM changed little within 5 weeks, then changed obviously and was 60% of the initial weight at the end of 12 weeks. The initial biomechanical strength of PDLLA was 1.33 MPa, decreased little within 3 weeks, then changed obviously and kept at 0.11 MPa at the end of 12 weeks. The initial biomechanical strength of PDLLA/HA/DBM was 1.7 MPa, decreased little within 4 weeks, then changed obviously and kept at 0.21 MPa at the end of 12 weeks.

CONCLUSIONS

The emulsion blend method is a new method to prepare bone repair materials. As a new bone repair material, PDLLA/HA/DBM is more suitable for regeneration and cell implantation, and the environment during its degradation is advantageous to the growth of bone cells.

摘要

目的

探讨DL-聚乳酸（PDLLA）/羟基磷灰石（HA）/脱钙骨基质（DBM）骨修复复合材料的制备方法，并研究其体外降解特性。

方法

采用乳液共混法制备PDLLA/HA/DBM复合材料，PDLLA∶HA∶DBM质量比为1.5 - 2∶1 - 1.5∶1。通过体外降解试验，分别研究PDLLA/HA/DBM和PDLLA在磷酸盐缓冲液（PBS，pH 7.4）中的重量、生物力学性能及pH值的动态变化。

结果

采用乳液共混法，无需加热即可将PDLLA、HA和DBM合成PDLLA/HA/DBM骨复合材料，该复合材料具有骨传导和骨诱导作用。孔径为100 - 400微米，孔隙率为71.3%。降解过程中，PDLLA溶液的pH值在2周内略有下降，但在4周结束时明显下降，值为4.0。而PDLLA/HA/DBM的pH值保持相当稳定，在12周结束时为6.4。PDLLA的重量在4周内变化不大，然后明显变化，在12周结束时为其初始重量的50%。而PDLLA/HA/DBM的重量在5周内变化不大，然后明显变化，在12周结束时为初始重量的60%。PDLLA的初始生物力学强度为1.33兆帕，在3周内下降不大，然后明显变化，在12周结束时保持在0.11兆帕。PDLLA/HA/DBM的初始生物力学强度为1.7兆帕，在4周内下降不大，然后明显变化，在12周结束时保持在0.21兆帕。