Tetik O, Islamoğlu F, Göncü T, Cekirdekçi A, Büket S
Department of Cardiovascular Surgery, Atatürk Medical Research Hospital, Izmir, Turkey.
Eur J Vasc Endovasc Surg. 2002 Dec;24(6):540-4. doi: 10.1053/ejvs.2002.1753.
to evaluate the effects of hypothermia and pentobarbital on spinal cord ischaemia induced in a rabbit model.
thirty-two rabbits, allocated into four equal groups, had the infrarenal aorta clamped distal to the left renal artery and above the iliac bifurcation for 40 min. Groups 3 and 4 had infusion of 15 mg/kg of pentobarbital intravenously for 5 min, 15 min before the cross-clamping. Groups 2 and 4 had infusion of 20 ml of Ringer's lactate (LR) solution at 3 degrees C for 3 min during aortic cross clamp into the isolated aortic segment. Group 1 was untreated and served as control. Postoperative functions of spinal cord were assessed.
paraplegia occurred in all rabbits in Group 1, in one in each of Groups 2 and 3, whereas no paraplegia was observed in Group 4. In addition 2 and 3 animals of Groups 2 and 3, respectively revealed varying degree of neurological disturbances, whereas all animals of Group 4 had normal function. This difference between Groups 2, 3, and 4 vs Group 1 was significant (p<0.002). So was the difference between Groups 2 and 4 (p=0.03), whereas the difference between Groups 3 and 4 was not significant.
hypothermia and pentobarbital was more effective than hypothermia alone for prevention of spinal cord ischaemia in a rabbit model.
评估低温和戊巴比妥对兔模型中脊髓缺血的影响。
32只兔子被平均分为四组，在左肾动脉远端和髂总动脉分叉上方夹闭肾下主动脉40分钟。第3组和第4组在夹闭前15分钟静脉注射15mg/kg戊巴比妥，持续5分钟。第2组和第4组在主动脉夹闭期间向分离的主动脉段输注20ml 3℃的乳酸林格氏液（LR），持续3分钟。第1组未接受治疗，作为对照组。评估术后脊髓功能。
第1组所有兔子均发生截瘫，第2组和第3组各有1只发生截瘫，而第4组未观察到截瘫。此外，第2组和第3组分别有2只和3只动物出现不同程度的神经功能障碍，而第4组所有动物功能正常。第2、3、4组与第1组之间的差异具有统计学意义（p<0.002）。第2组和第4组之间的差异也具有统计学意义（p=0.03），而第3组和第4组之间的差异无统计学意义。
在兔模型中，低温联合戊巴比妥在预防脊髓缺血方面比单纯低温更有效。
