与罗-琴培养基相比，评估MB/BacT系统从临床标本中分离分枝杆菌的能力。

Evaluation of the MB/BacT system for recovery of mycobacteria from clinical specimens in comparison to Lowenstein-Jensen medium.

作者信息

Mirovic V, Lepsanovic Z

机构信息

Department of Microbiology, Military Medical Academy, Belgrade, Yugoslavia.

出版信息

Clin Microbiol Infect. 2002 Nov;8(11):709-14. doi: 10.1046/j.1469-0691.2002.00448.x.

DOI:10.1046/j.1469-0691.2002.00448.x

PMID:12445008

Abstract

OBJECTIVE

To evaluate the performance of the MB/BacT system (Organon Teknika) in comparison to Lowenstein-Jensen (LJ) solid medium for recovery of mycobacteria from clinical specimens.

METHODS

Two thousand three hundred and ten specimens (1626 respiratory, 593 urine, 60 body fluids, five tissue and 26 others) were inoculated in MB/BacT (0.5 mL) and on two LJ slants (0.25 mL each). N-acetyl-l-cysteine-NaOH (final concentration 2%) was used for decontamination.

RESULTS

Two hundred and fifty-one (10.8%) mycobacterial isolates [190 Mycobacterium tuberculosis complex (MTBC) and 61 non-tuberculous mycobacteria (NTM)] were detected. Of these 251 isolates, 234 (93.2%; 181 MTBC and 53 NTM) were detected in MB/BacT and 169 (67.3%; 154 MTBC and 15 NTM) on LJ. The mean (median) times to detection of MTBC by MB/BacT and LJ were 13.8 (13) and 22.1 (20) days, respectively, while overall contamination rates were 7.7% and 8.1%, respectively.

CONCLUSIONS

Sensitivity and time to detection were significantly better with MB/BacT than with solid LJ medium.

摘要

目的

评估MB/BacT系统（奥加农公司）与罗氏（LJ）固体培养基相比，从临床标本中分离分枝杆菌的性能。

方法

将2310份标本（1626份呼吸道标本、593份尿液标本、60份体液标本、5份组织标本和26份其他标本）接种于MB/BacT系统（0.5 mL）和两支LJ斜面培养基（各0.25 mL）。使用N-乙酰-L-半胱氨酸-氢氧化钠（终浓度2%）进行去污处理。

结果

共检测到251株（10.8%）分枝杆菌分离株[190株结核分枝杆菌复合群（MTBC）和61株非结核分枝杆菌（NTM）]。在这251株分离株中，MB/BacT系统检测到234株（93.2%；181株MTBC和53株NTM），LJ培养基检测到169株（67.3%；154株MTBC和15株NTM）。MB/BacT系统和LJ培养基检测到MTBC的平均（中位数）时间分别为13.8（13）天和22.1（20）天，总体污染率分别为7.7%和8.1%。