Swelsen W T N, Voorter C E M, van den Berg-Loonen E M
Tissue Typing Laboratory, University Hospital Maastricht, The Netherlands.
Tissue Antigens. 2002 Sep;60(3):224-34. doi: 10.1034/j.1399-0039.2002.600304.x.
The HLA-B5/35 cross-reacting group (CREG) is a set of closely related antigens including HLA-B35, B51, B52, B53 and B78. The nucleotide sequences of exon 1 through 5 of the B5/35 CREG were determined to assess the level of polymorphism. For exons 2 and 3, the previously described sequence-based typing (SBT) strategy was applied, the nucleotide sequences of exon 1, 4 and 5 were determined by allele-specific sequencing. A total of 225 unrelated individuals were HLA-B typed by heterozygous sequencing of exons 2 and 3. In the B5/35 CREG, 26 different alleles were identified, whereas 63 non-B5/35 CREG alleles were sequenced. The SBT strategy was proven to be reliable and efficient for high resolution typing of the B5/35 CREG. The nucleotide sequences of exon 1, 4 and 5 were determined for the 26 different B5/35 CREG alleles to establish the level of polymorphism. For seven different alleles, of which the exon 1, 4 and 5 sequences were hitherto unknown, the sequences were elucidated and in agreement with the known B5/35 sequences. Nineteen HLA-B5/35 CREG alleles with previously published exon 1, 4 and 5 sequences were sequenced in at least two individuals. Three new alleles were identified. The first, B5204, showed a difference at position 200 compared to B52011, which was previously considered a conserved position. The other two alleles, B3542 and B51015, showed exon 2 and 3 sequences identical to B35011 and B51011, but differences in exons 1 and 4, respectively. B3542 had differences at position 25 and 72 and B51015 showed a difference at position 636. More polymorphism might be present outside exons 2 and 3 than previously thought.
HLA - B5/35交叉反应组（CREG）是一组密切相关的抗原，包括HLA - B35、B51、B52、B53和B78。测定了B5/35 CREG第1至5外显子的核苷酸序列，以评估多态性水平。对于第2和第3外显子，应用了先前描述的基于序列的分型（SBT）策略，第1、4和5外显子的核苷酸序列通过等位基因特异性测序确定。通过对第2和第3外显子进行杂合测序，对总共225名无关个体进行了HLA - B分型。在B5/35 CREG中，鉴定出26个不同的等位基因，同时对63个非B5/35 CREG等位基因进行了测序。结果证明，SBT策略对于B5/35 CREG的高分辨率分型是可靠且有效的。测定了26个不同的B5/35 CREG等位基因第1、4和5外显子的核苷酸序列，以确定多态性水平。对于7个不同的等位基因，其第1、4和5外显子的序列此前未知，现已阐明其序列且与已知的B5/35序列一致。对19个具有先前公布的第1、4和5外显子序列的HLA - B5/35 CREG等位基因，至少在两名个体中进行了测序。鉴定出3个新等位基因。第一个是B5204，与先前被认为是保守位置的B52011相比，在第200位存在差异。另外两个等位基因B3542和B51015，其第2和第3外显子序列分别与B35011和B51011相同，但第1和第4外显子存在差异。B3542在第25和72位存在差异，B51015在第636位存在差异。第2和第3外显子之外可能存在比先前认为的更多的多态性。
