Celik Ilhami, Akbulut Ayhan, Kilic S Sirri, Rahman Ali, Vural Pervin, Canbaz Mukaddes, Felek Suleyman
Department of Clinical Microbiology and Infectious Diseases, College of Medicine, Firat University, Elazig, Turkey.
BMC Infect Dis. 2002 Oct 31;2:26. doi: 10.1186/1471-2334-2-26.
Despite major developments in the management of septic shock, the mortality rate had progressively increased. Ibuprofen has been shown to have beneficial physiological effects when used as a treatment. However, there are conflicting results with respect to survival. This study aims to investigate the effect of ibuprofen on vital functions, various physiological parameters and survival during endotoxic shock in rabbits.
Twenty-eight New Zealand rabbits were randomly separated into four groups. The first group received only saline, the second was given 2 mg/kg intravenous endotoxin at t0, the third received 30 mg/kg ibuprofen 30 minutes after endotoxin administration, whilst the fourth group received ibuprofen 30 minutes before the endotoxin. Respiratory and heart rate, mean arterial blood pressure and rectal temperature were recorded. Complete blood counts were performed and thromboxane B2 was measured every 30 minutes for the first two hours, and then hourly over the course of the experiment. Urine samples were collected at the same time points for the measurement of prostaglandin E2.
Ibuprofen was found to improve respiratory rate, heart rate, and arterial pressure. However, it did not improve the negative effects of endotoxin on body temperature, haematocrit values, white blood cell count, and thrombocyte number. Thromboxane B2 levels in group IV were significantly lower than in the other groups, and the increase started at a later timepoint. In ibuprofen-treated animals, Prostaglandin E2 levels stayed low for at least 90 minutes, but started to rise thereafter. While the average survival in Group II animals was 192.9 +/- 46.9 minutes, those of groups III and IV were 339.1 +/- 33.5 minutes (p < 0.05) and 383.0 +/- 39.6 minutes (p = 0.01), respectively.
Ibuprofen appears to increase survival in endotoxic shock-induced animals. Therefore, it may be helpful for the prophylaxis and treatment of patients with, or who are likely to develop, septic shock.
尽管感染性休克的治疗取得了重大进展，但其死亡率却在逐渐上升。布洛芬在用作治疗时已显示出有益的生理效应。然而，关于其对生存率的影响存在相互矛盾的结果。本研究旨在探讨布洛芬对内毒素休克家兔重要生命功能、各种生理参数及生存率的影响。
将28只新西兰家兔随机分为四组。第一组仅给予生理盐水，第二组在t0时刻静脉注射2mg/kg内毒素，第三组在内毒素给药30分钟后给予30mg/kg布洛芬，而第四组在内毒素给药前30分钟给予布洛芬。记录呼吸和心率、平均动脉血压及直肠温度。在前两小时内每30分钟进行一次全血细胞计数并测量血栓素B2，然后在实验过程中每小时测量一次。在相同时间点收集尿液样本以测量前列腺素E2。
发现布洛芬可改善呼吸频率、心率和动脉血压。然而，它并未改善内毒素对体温、血细胞比容值、白细胞计数和血小板数量的负面影响。第四组的血栓素B2水平显著低于其他组，且其升高开始时间较晚。在布洛芬治疗的动物中，前列腺素E2水平至少在90分钟内保持较低，但此后开始上升。第二组动物的平均生存时间为192.9±46.9分钟，而第三组和第四组分别为339.1±33.5分钟（p<0.05）和383.0±39.6分钟（p = 0.01）。
布洛芬似乎可提高内毒素休克诱导动物的生存率。因此，它可能有助于预防和治疗感染性休克患者或可能发生感染性休克的患者。
