Akbulut Handan, Celik Ilhami, Ayar Ahmet, Vural Pervin, Canbaz Mukaddes
Department of Immunology, University of Firat, Faculty of Medicine, Elazig, Turkey.
Neuro Endocrinol Lett. 2005 Aug;26(4):407-12.
The purpose of this study was to investigate the effects of ibuprofen on plasma nitrite/nitrate levels, as indirect indicators of nitric oxide, in correlation with blood pressure in a rabbit model of endotoxin-induced shock.
A total of 28 rabbits were randomly divided into four groups. Control group received physiological saline, while endotoxin (ETX, E. Coli, 055:B5, 2 mg/kg, i.v.) was administered to the rabbits in the other groups: group II receiving only ETX, in addition to ETX group III received ibuprofen (30 mg/kg) 30 minutes after ETX administration, whilst the group IV received ibuprofen (30 mg/kg) 30 minutes before ETX. Arterial blood pressure and plasma levels of nitrite/nitrate were determined immediately before (time 0) and 30, 60, 90, 120, 180, 240, and 300 minutes after ETX administration.
ETX administered groups had significantly higher plasma levels of nitrite/nitrate than the control group, at all consecutive measurements except at time O. Treatment with ibuprofen, either before or after ETX, partly restored the elevated levels of nitrite/nitrate. ETX also caused a significant decrease in blood pressure which was prevented in ibuprofen treated groups.
Results from this study indicate that administration of ibuprofen prevents sudden reductions in blood pressure by inhibiting excessive production of nitric oxide in rabbit model of endotoxin-induced shock and this may be of importance for providing crucial time for therapeutic intervention and survival in septic shock.
本研究旨在探讨布洛芬对内毒素诱导休克兔模型中血浆亚硝酸盐/硝酸盐水平（作为一氧化氮的间接指标）的影响，并与血压进行相关性研究。
总共28只兔子被随机分为四组。对照组接受生理盐水，而其他组的兔子接受内毒素（ETX，大肠杆菌，055:B5，2mg/kg，静脉注射）：第二组仅接受ETX，第三组在ETX给药30分钟后除ETX外还接受布洛芬（30mg/kg），而第四组在ETX给药前30分钟接受布洛芬（30mg/kg）。在ETX给药前（时间0）以及给药后30、60、90、120、180、240和300分钟测定动脉血压和血浆亚硝酸盐/硝酸盐水平。
除时间0外，在所有连续测量中，接受ETX的组血浆亚硝酸盐/硝酸盐水平均显著高于对照组。在ETX之前或之后用布洛芬治疗，部分恢复了亚硝酸盐/硝酸盐的升高水平。ETX还导致血压显著下降，而布洛芬治疗组可预防这种情况。
本研究结果表明，在兔内毒素诱导休克模型中，布洛芬给药可通过抑制一氧化氮的过度产生来防止血压突然下降，这对于为脓毒性休克的治疗干预和生存提供关键时间可能具有重要意义。
