Bao Shunzhong, Rai Jodie, Schreiber James
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
J Soc Gynecol Investig. 2002 Nov-Dec;9(6):351-6.
To determine whether nitric oxide synthase isoforms are expressed in human myometrium, to define their localization, and to examine the possibility that one or more may play a role in initiating labor.
Human myometrial biopsies were collected from term laboring and nonlaboring patients. The mRNA expression was assessed using in situ hybridization. The relative expression levels were also assessed by Affymetrix GeneChip hybridization. The protein expression was determined using Western blot analysis, and localization was shown using immunostaining.
Both inducible nitric oxide synthase and brain nitric oxide synthase were expressed in myometrial cells and blood vessel endothelial cells. Endothelial nitric oxide synthase was expressed exclusively in blood vessel endothelial cells. There were no differences in distribution or quantity of nitric oxide synthase expression between term labor and nonlabor myometrium.
All three isotypes of nitric oxide synthases were expressed in human pregnant term uterus. Their expression did not change in the myometrium of the laboring uterus.
确定一氧化氮合酶亚型是否在人子宫肌层中表达，明确其定位，并探讨一种或多种亚型在发动分娩中发挥作用的可能性。
从足月分娩和未分娩患者中采集人子宫肌层活检组织。采用原位杂交评估mRNA表达。还通过Affymetrix基因芯片杂交评估相对表达水平。使用蛋白质印迹分析确定蛋白质表达，并通过免疫染色显示定位。
诱导型一氧化氮合酶和脑型一氧化氮合酶均在子宫肌层细胞和血管内皮细胞中表达。内皮型一氧化氮合酶仅在血管内皮细胞中表达。足月分娩子宫肌层和未分娩子宫肌层中一氧化氮合酶表达的分布或数量无差异。
一氧化氮合酶的所有三种亚型均在人妊娠足月子宫中表达。它们在分娩子宫肌层中的表达没有变化。
