Hefler Lukas A, Ludwig Elisabeth, Lebrecht Antje, Zeillinger Robert, Tong-Cacsire Dan, Koelbl Heinz, Leodolter Sepp, Tempfer Clemens B
Department of Obstetrics and Gynecology, University of Vienna Medical School, Vienna, Austria.
J Soc Gynecol Investig. 2002 Nov-Dec;9(6):386-90.
Interleukin-1 (IL-1) is known to be critically involved in ovarian carcinogenesis. We investigated a panel of polymorphisms of the IL-1 and the IL-1 receptor antagonist (IL-1 RA) genes in patients with ovarian cancer.
One hundred thirty-four patients with surgically staged ovarian cancer, 27 patients with borderline ovarian cancer, and 134 healthy controls were genotyped for three polymorphisms of the IL-1 gene (-889 C/T polymorphism of the IL-1alpha gene [IL1A], -511 C/T polymorphism of the IL-1beta promoter [IL1B promoter], a polymorphism of IL-1beta exon 5 [IL1B exon 5]), and an 86-base pair repeat in intron 2 of the IL-1 RA gene [IL1RN]) using polymerase chain reaction and pyrosequencing.
Allelic frequencies did not differ between patients with ovarian cancer and controls. In the ovarian cancer group, polymorphism did not correlate with any of the investigated clinicopathologic variables, including tumor stage, lymph node, and histologic grade. In univariate and multivariate models, there was no correlation between any polymorphism and patients' overall and disease-free survival.
We investigated interleukin polymorphisms in ovarian cancer but did not find any association between common polymorphisms of IL1A, IL1B, and IL1RN and the occurrence of ovarian cancer. Allelic variation within the IL-1 gene clusters does not seem to play a role in ovarian carcinogenesis and does not appear to be a useful tool for possible screening and risk evaluation.
已知白细胞介素-1（IL-1）在卵巢癌发生过程中起关键作用。我们研究了卵巢癌患者中IL-1及IL-1受体拮抗剂（IL-1RA）基因的一组多态性。
对134例经手术分期的卵巢癌患者、27例卵巢交界性肿瘤患者及134例健康对照者，采用聚合酶链反应和焦磷酸测序技术，对IL-1基因的3种多态性（IL-1α基因的-889C/T多态性[IL1A]、IL-1β启动子的-511C/T多态性[IL1B启动子]、IL-1β外显子5的多态性[IL1B外显子5]）以及IL-1RA基因内含子2中的一个86碱基对重复序列[IL1RN]进行基因分型。
卵巢癌患者与对照者的等位基因频率无差异。在卵巢癌组中，多态性与任何所研究的临床病理变量均无相关性，包括肿瘤分期、淋巴结及组织学分级。在单因素和多因素模型中，任何多态性与患者的总生存期和无病生存期均无相关性。
我们研究了卵巢癌中的白细胞介素多态性，但未发现IL1A、IL1B和IL1RN的常见多态性与卵巢癌的发生之间存在任何关联。IL-1基因簇内的等位基因变异似乎在卵巢癌发生过程中不起作用，也似乎不是用于可能的筛查和风险评估的有用工具。
