Fukushima N, Yokouchi K, Kawagishi K, Moriizumi T
Department of Anatomy, Shinshu University School of Medicine, Matsumoto 390-8621, Japan.
Neurosci Res. 2002 Dec;44(4):467-73. doi: 10.1016/s0168-0102(02)00173-6.
The rostral migratory stream (RMS) is a unique forebrain structure that provides a long-distance migratory route for the neural stem cells of the periventricular region towards the olfactory bulb (OB). The purpose of the study presented here is to examine the extent of neurogenesis and gliogenesis by the neural stem cells of different origins (periventricular vs. intrabulbar) in the OB. After the RMS had been subjected to injury, the rats received intraperitoneal injections of 5-bromodeoxyuridine (BrdU) and were further reared for 2 weeks. Neuronal and glial differentiations of the BrdU(+) cells in the olfactory bulbar granule cell (OB-GCL) and the olfactory glomerular (OB-GL) layers were examined immunohistochemically using antibodies against neuronal (NeuN, neuronal nuclei) and glial (GFAP, glial fibrillary acidic protein) markers in the OBs with injured and uninjured (control) RMS. In the completely RMS-lesioned OB, where migration of the periventricular neural stem cells was inhibited, a small number of BrdU(+) NeuN(+) cells were found in both the OB-GCL and OB-GL. The BrdU(+) NeuN(+) cells accounted for a much higher percentage of the BrdU(+) cells on the control side (OB-GCL, 36.7%; OB-GL, 8.8%) than on the completely RMS-lesioned side (OB-GCL, 3.7%; OB-GL, 0.6%). The percentage of the BrdU(+) GFAP(+) cells relative to the BrdU(+) cells did not show any major difference between the control and completely RMS-lesioned sides. This study revealed differences in neurogenesis and gliogenesis between the local and migrating neural stem cells in the OB of the adult rodent.
吻侧迁移流（RMS）是一种独特的前脑结构，为脑室周围区域的神经干细胞向嗅球（OB）提供了一条长距离迁移途径。本文所述研究的目的是检测OB中不同来源（脑室周围与球内）的神经干细胞的神经发生和胶质发生程度。在RMS受到损伤后，给大鼠腹腔注射5-溴脱氧尿苷（BrdU），并进一步饲养2周。使用针对OB中神经元（NeuN，神经元细胞核）和胶质细胞（GFAP，胶质纤维酸性蛋白）标志物的抗体，通过免疫组织化学方法检测嗅球颗粒细胞层（OB-GCL）和嗅小球层（OB-GL）中BrdU(+)细胞的神经元和胶质细胞分化情况，其中一部分OB的RMS受到损伤，另一部分未受损伤（作为对照）。在完全RMS损伤的OB中，脑室周围神经干细胞的迁移受到抑制，在OB-GCL和OB-GL中均发现少量BrdU(+)NeuN(+)细胞。与对照侧（OB-GCL为36.7%；OB-GL为8.8%）相比，BrdU(+)NeuN(+)细胞在完全RMS损伤侧（OB-GCL为3.7%；OB-GL为0.6%）的BrdU(+)细胞中所占百分比要低得多。相对于BrdU(+)细胞，BrdU(+)GFAP(+)细胞的百分比在对照侧和完全RMS损伤侧之间未显示出任何显著差异。这项研究揭示了成年啮齿动物OB中局部和迁移神经干细胞在神经发生和胶质发生方面的差异。
