蜡样芽孢杆菌产生的肠毒素T（BcET）可能不会导致食物中毒。
The enterotoxin T (BcET) from Bacillus cereus can probably not contribute to food poisoning.
作者信息
Choma Caroline, Granum Per Einar
机构信息
Norwegian School of Veterinary Science, Department of Pharmacology Microbiology and Food Hygiene, PO Box 8146, 0033, Oslo, Norway.
出版信息
FEMS Microbiol Lett. 2002 Nov 19;217(1):115-9. doi: 10.1111/j.1574-6968.2002.tb11464.x.
A polymerase chain reaction (PCR) fragment from strain NVH 38 (containing bceT) was cloned, sequenced and expressed in Escherichia coli. This sequence showed 50-60% identity to the original. When this bceT clone was expressed in E. coli no biological activity was found in either supernatants or cell extracts. Cell extracts from the Bacillus cereus strains (NVH 38 and B-4ac) were also negative on Vero cells. Neutralisation of supernatant from B. cereus B-4ac using a monoclonal antibody (reacting with NheB and HblD) abolished the activity. A test for cytotoxic enterotoxins was negative for both cell extracts and supernatants. Our data suggest that BcET either has an unknown type enterotoxic action or non at all.
来自NVH 38菌株（含有bceT）的聚合酶链反应（PCR）片段被克隆、测序并在大肠杆菌中表达。该序列与原始序列的同一性为50-60%。当这个bceT克隆在大肠杆菌中表达时，在上清液或细胞提取物中均未发现生物活性。蜡样芽孢杆菌菌株（NVH 38和B-4ac）的细胞提取物对Vero细胞也呈阴性。使用单克隆抗体（与NheB和HblD反应）中和蜡样芽孢杆菌B-4ac的上清液可消除活性。细胞提取物和上清液的细胞毒性肠毒素检测均为阴性。我们的数据表明，BcET要么具有未知类型的肠毒素作用，要么根本没有。
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