Fernández-Guasti A, del Río Portilla I Y, Ugalde E, Corsi-Cabrera M
Departamento de Farmacobiología, CINVESTAV, Calzada de los Tenorios 235, Col. Granjas Coapa, Mexico City, 14330 D.F., Mexico.
Psychoneuroendocrinology. 2003 Jan;28(1):85-100. doi: 10.1016/s0306-4530(02)00011-2.
This study was designed to analyse if the effect of diazepam or progesterone on the wakeful rat EEG vary according to sex and neonatal sexual differentiation. Diazepam or progesterone was administered to males, females, neonatally castrated males and neonatally virilised females. Comparisons of drug actions were made versus baseline values. Both compounds produced clear sexual dimorphic responses, males being more sensitive than females. In normal males and neonatally virilised females diazepam produced a clear increase in the absolute power of fast frequencies. In normal females and neonatally castrated males this anxiolytic only produced a moderate increase in the absolute power of the beta 2 band. Regarding interparietal correlation, diazepam produced an increase in males in the alpha, beta 1 and beta 2 bands, while in females this drug caused a reduction in the same bands. Neonatally castrated males or virilised females showed an intermediate pattern. These data suggest that sexual dimorphism in diazepam action depends upon neonatal sexual differentiation. Progesterone, in contrast with diazepam, produced effects on the EEG that were also sexually dimorphic but independent of the sexual differentiation process. This steroid in males induced an increase in the absolute power of the fast bands of the EEG (alpha, beta 1 and beta 2) accompanied by an increased interparietal correlation of the alpha band. In females, progesterone only reduced the interparietal correlation in the fast theta and alpha bands. Data show similarities between diazepam and progesterone effects on the EEG that are discussed in the light of the anxiolytic-like and hypnotic properties of these compounds. A similar mechanism of action for both substances, involving the GABA(A) receptor, is sustained.
本研究旨在分析地西泮或孕酮对清醒大鼠脑电图的影响是否因性别和新生儿期性分化而异。将地西泮或孕酮分别给予雄性、雌性、新生期去势雄性和新生期雄性化雌性大鼠。将药物作用与基线值进行比较。两种化合物均产生明显的性别二态性反应，雄性比雌性更敏感。在正常雄性和新生期雄性化雌性大鼠中，地西泮使快速频率的绝对功率明显增加。在正常雌性和新生期去势雄性大鼠中，这种抗焦虑药仅使β2频段的绝对功率适度增加。关于顶叶间相关性，地西泮使雄性大鼠的α、β1和β2频段增加，而在雌性大鼠中，该药使相同频段降低。新生期去势雄性或雄性化雌性大鼠表现出中间模式。这些数据表明，地西泮作用的性别二态性取决于新生儿期性分化。与地西泮相反，孕酮对脑电图的影响也具有性别二态性，但与性分化过程无关。这种类固醇在雄性大鼠中诱导脑电图快速频段（α、β1和β2）的绝对功率增加，同时α频段的顶叶间相关性增加。在雌性大鼠中，孕酮仅降低快速θ和α频段的顶叶间相关性。数据显示了地西泮和孕酮对脑电图影响的相似性，并根据这些化合物的抗焦虑样和催眠特性进行了讨论。两种物质涉及GABA(A)受体的类似作用机制得到了支持。
