Chénard Gilles, Miedema Kor, Moonen Peter, Schrijver Remco S, Dekker Aldo
ID-Lelystad, Institute for Animal Science and Health, PO Box 65, 8200 AB, Lelystad, The Netherlands.
J Virol Methods. 2003 Jan;107(1):89-98. doi: 10.1016/s0166-0934(02)00196-9.
A simple solid-phase blocking ELISA for the detection of antibodies directed against type O foot-and-mouth disease virus (FMDV) was developed. The ELISA was validated using field sera collected from cattle, pigs and sheep originating from FMDV infected and non-infected Dutch farms, reference sera obtained from the World Reference Laboratory for foot-and-mouth disease at the Institute for Animal Health, Pirbright Laboratory, UK and sera from experimentally infected animals. Testing 2664 sera collected from non-infected cattle, pigs and sheep resulted in a specificity of 96%. A sensitivity relative to the virus neutralisation test (VNT) of >99% was achieved when testing 148 positive cattle, goat and sheep sera collected from FMDV-infected Dutch farms. All international reference sera scored consistently correct. The ELISA also correctly scored 398 of 409 positive experimentally derived sera. The sensitivity and specificity of this monoclonal antibody-based ELISA for detection of type O FMDV antibodies is sufficient for use as a screening ELISA. During the 2001 epidemic in the Netherlands, 8000 serum samples per day were regularly tested in this ELISA. The samples scoring positive were then tested by neutralisation for confirmation thus making optimum use of the neutralisation testing capacity.
开发了一种用于检测抗O型口蹄疫病毒（FMDV）抗体的简单固相阻断ELISA。使用从荷兰感染和未感染FMDV的农场采集的牛、猪和羊的现场血清、从英国皮尔布赖特实验室动物卫生研究所口蹄疫世界参考实验室获得的参考血清以及实验感染动物的血清对该ELISA进行了验证。对从未感染的牛、猪和羊采集的2664份血清进行检测，特异性为96%。对从荷兰感染FMDV的农场采集的148份阳性牛、山羊和绵羊血清进行检测时，相对于病毒中和试验（VNT）的灵敏度>99%。所有国际参考血清的评分均一致正确。该ELISA对409份实验来源的阳性血清中的398份也进行了正确评分。这种基于单克隆抗体的ELISA检测O型FMDV抗体的灵敏度和特异性足以用作筛查ELISA。在2001年荷兰疫情期间，每天有8000份血清样本定期用该ELISA进行检测。对评分呈阳性的样本随后进行中和试验以确认，从而最佳地利用了中和试验能力。
