Iwakami Tomohiko, Imai Toshio
Department of Anatomy, School of Dentistry at Tokyo, The Nippon Dental University, Fujimi Chiyida-ku, Tokyo 102-8159, Japan.
Biomed Mater Eng. 2002;12(3):249-57.
The effect of hydroxyapatite sol (HAm) which is composed of unheating hydroxyapatite microcrystal particle on cell proliferation and alkaline phophatase activity in osteoblastic MC3T3-E1 cells, was examined in the presence or absence of fetal calfe serum (FCS) in the medium. In the absence of FCS, HAm inhibited the cell proliferation of MC3T3-E1 cells in a dose-dependent manner, but not in the presence of FCS. Inhibitory effect by HAm was observed only in directly contact with the cells. Similar inhibition effects by HAm were also observed on ALP activity of the cells. However, inhibitory effect by HAm on ALP extracted from the cells was not observed. After HAm treatment, ALP activity in the medium was increased. HAm pretreated with FCS did not inhibit the ALP activity of the cells at all. These results suggest that serum may play an important role in the biocompatibility of HAm and osteoblastic cells.
研究了由未加热的羟基磷灰石微晶颗粒组成的羟基磷灰石溶胶（HAm）在培养基中存在或不存在胎牛血清（FCS）的情况下，对成骨细胞MC3T3-E1细胞增殖和碱性磷酸酶活性的影响。在没有FCS的情况下，HAm以剂量依赖性方式抑制MC3T3-E1细胞的增殖，但在有FCS的情况下则不然。仅在与细胞直接接触时观察到HAm的抑制作用。在细胞的碱性磷酸酶活性上也观察到了HAm的类似抑制作用。然而，未观察到HAm对从细胞中提取的碱性磷酸酶的抑制作用。HAm处理后，培养基中的碱性磷酸酶活性增加。用FCS预处理的HAm根本不抑制细胞的碱性磷酸酶活性。这些结果表明，血清可能在HAm与成骨细胞的生物相容性中起重要作用。
