Yada M, Yamaguchi K, Tsuji T
Sagami Chemical Research Center, Kanagawa, Japan.
Biochem Biophys Res Commun. 1994 Dec 15;205(2):1187-93. doi: 10.1006/bbrc.1994.2791.
The activities of NGF on induction of IL-6, ALP and collagen were studied in cultured osteoblastic cells, MC3T3-E1. Treatment of the cells with NGF induced IL-6 production in a dose dependent manner and enhanced ALP activity and collagen biosynthesis without affecting cell proliferation. Cyclo-oxygenase was activated in NGF-treated cells and prostaglandinE2 was detected as a major metabolite from arachidonic acid. Since prostaglandinE2 is an enhancer of ALP activity and collagen biosynthesis in MC3T3-E1 and was found to induce IL-6 production in this study, it is possible that NGF works on MC3T3-E1 cells through the mediation by endogenous prostaglandinE2.
在培养的成骨细胞MC3T3-E1中研究了神经生长因子（NGF）对白细胞介素-6（IL-6）、碱性磷酸酶（ALP）和胶原蛋白的诱导作用。用NGF处理细胞以剂量依赖性方式诱导IL-6产生，并增强ALP活性和胶原蛋白生物合成，而不影响细胞增殖。在经NGF处理的细胞中环氧化酶被激活，前列腺素E2被检测为花生四烯酸的主要代谢产物。由于前列腺素E2是MC3T3-E1中ALP活性和胶原蛋白生物合成的增强剂，且在本研究中发现其可诱导IL-6产生，因此NGF可能通过内源性前列腺素E2的介导作用于MC3T3-E1细胞。
