Greene Catherine, Lowe Gregory, Taggart Clifford, Gallagher Paula, McElvaney Noel, O'Neill Shane
Respiratory Research Division, Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland.
J Infect Dis. 2002 Dec 15;186(12):1790-6. doi: 10.1086/345799. Epub 2002 Nov 14.
Bronchoalveolar lavage fluid recovered from infected and uninvolved lungs of patients with community-acquired pneumonia (CAP; n=16) on day 6+/-0.8 was analyzed for cytokine, soluble receptor, and antagonist levels. The role of tumor necrosis factor (TNF)-alpha-converting enzyme (TACE) in the resolution of the local inflammatory response was investigated. TNF-alpha, interleukin (IL)-1beta, and IL-6 were elevated in the infected versus uninvolved lobe, whereas IL-10 was not. Epithelial lining fluid (ELF) cytokine levels correlated with intracellular cytokine expression. Levels of proTNF-alpha were reciprocally related to TNF-alpha ELF levels. Levels of soluble receptors, generated by TACE cleavage of membrane-bound precursors, were compartmentalized to infected ELF. TACE was down-regulated by internalization in cells from the site of infection. These data demonstrate that, in vivo during CAP, TACE has a role in regulating resolution of the local inflammatory response by modulating levels of pro- and counterinflammatory mediators.
对16例社区获得性肺炎（CAP）患者在第6天±0.8天从感染肺叶和未感染肺叶回收的支气管肺泡灌洗液进行细胞因子、可溶性受体和拮抗剂水平分析。研究了肿瘤坏死因子（TNF）-α转换酶（TACE）在局部炎症反应消退中的作用。与未感染肺叶相比，感染肺叶中的TNF-α、白细胞介素（IL）-1β和IL-6升高，而IL-10未升高。上皮衬液（ELF）细胞因子水平与细胞内细胞因子表达相关。前TNF-α水平与TNF-α ELF水平呈负相关。由TACE切割膜结合前体产生的可溶性受体水平在感染的ELF中分隔分布。TACE在感染部位的细胞中通过内化而下调。这些数据表明，在CAP患者体内，TACE通过调节促炎和抗炎介质水平在调节局部炎症反应的消退中发挥作用。
