Light J A, Annable C, Perloff L J, Sulkin M D, Hill G S, Etheredge E E, Spees E K
Transplantation. 1975 Jun;19(6):511-6.
Two pairs of plasma-perfused human cadaver kidneys were rejected in a hyperacute manner by recipients who had not previously received a transplant. Crossmatches between recipient sera and donor lymphocytes were negative in all cases. A fifth kidney was plasma-perfused but not transplanted because the perfusate was shown to be cytotoxic to donor lymphocytes. IgM and complement, but not IgG, were demonstrated in these kidneys by immunofluorescent microscopy and confirmed by further immunological studies. The IgM was broadly reactive against multiple HL-A specificities and was present in 11 percent of sera from normal, healthy male donors. It appears from our studies that cytotoxic IgM may be present in homologous plasma and cause immune injury to the kidney during ex vivo pulsatile preservation. This may be responsible for some cases of otherwise unexplained accelerated allograft rejection.
两对经血浆灌注的人尸体肾脏被未曾接受过移植的受者以超急性方式排斥。所有病例中，受者血清与供者淋巴细胞的交叉配型均为阴性。第五个肾脏经血浆灌注但未移植，因为灌注液被证明对供者淋巴细胞具有细胞毒性。通过免疫荧光显微镜在这些肾脏中检测到了IgM和补体，但未检测到IgG，并通过进一步的免疫学研究得到证实。该IgM对多种HL - A特异性具有广泛反应性，在正常健康男性供者的11%的血清中存在。我们的研究表明，细胞毒性IgM可能存在于同源血浆中，并在体外搏动保存期间对肾脏造成免疫损伤。这可能是一些原因不明的加速性同种异体移植排斥反应病例的原因。
