Stewart R J, Askew E W, McDonald C M, Metos J, Jackson W D, Balon T W, Prior R L
St Luke's Wood River Medical Center, Ketchum, ID, USA.
J Am Diet Assoc. 2002 Nov;102(11):1652-7. doi: 10.1016/s0002-8223(02)90352-4.
To study oxidative stress indicators in healthy young children and their response to a commercially available fruit- and vegetable-based antioxidant supplement.
Healthy children were randomly assigned to a placebo and a supplement (commercial antioxidant supplement produced from dried fruit and vegetable extracts and fortified with antioxidants, resembling a gummy-type candy). The placebo and the supplement were taken in 2 doses per day for 21 days.
Participants were 39 children (26 boys and 13 girls) aged 5 to 10 years. Research was conducted at Primary Children's Medical Center and the University of Utah, Salt Lake City.
Breath and urine samples were collected on days 1 and 21 and assayed for breath pentane and urine 8-hydroxydeoxyguanosine, malondialdehyde, nitrites, and 8-isoprostane as noninvasive indicators of oxidative stress. Urine oxygen radical absorbance capacity was measured at days 1 and 21 as an indirect indicator of the antioxidant capacity of the body. Three-day food records were collected at the beginning and end of the study to measure intake of dietary fruit; vegetable; and antioxidant vitamins A, C, and E.
Descriptive statistics, repeated measures analysis of variance, paired t tests, and Pearson r correlations.
Markers of oxidative stress were not significantly different between the placebo and supplement groups at day 1 or day 21. The oxidative stress indicators of the healthy children in this study appear to be similar to those of healthy adults and were not changed by antioxidant supplementation. The diet record analyses indicated that mean fruit and vegetable intakes (2.75 servings/day) were similar to the national average intake for children in the United States.
APPLICATIONS/CONCLUSIONS: This research presents original information on the subject of oxidative stress in healthy children. The results of this study may be useful as reference baseline markers to use in conjunction with clinical dietary evaluations and for future research with healthy children and with children in disease states who are subject to elevated levels of oxidative stress.
研究健康幼儿的氧化应激指标及其对市售水果和蔬菜基抗氧化剂补充剂的反应。
将健康儿童随机分为安慰剂组和补充剂组（由干果蔬提取物制成并添加抗氧化剂的市售抗氧化剂补充剂，类似软糖型糖果）。安慰剂和补充剂每天服用2剂，共服用21天。
39名年龄在5至10岁的儿童（26名男孩和13名女孩）。研究在盐湖城的 Primary Children's Medical Center 和犹他大学进行。
在第1天和第21天采集呼吸和尿液样本，检测呼吸戊烷、尿液8-羟基脱氧鸟苷、丙二醛、亚硝酸盐和8-异前列腺素，作为氧化应激的非侵入性指标。在第1天和第21天测量尿液氧自由基吸收能力，作为身体抗氧化能力的间接指标。在研究开始和结束时收集3天的食物记录，以测量膳食水果、蔬菜以及抗氧化维生素A、C和E的摄入量。
描述性统计、重复测量方差分析、配对t检验和Pearson r相关性分析。
在第1天或第21天，安慰剂组和补充剂组之间的氧化应激标志物无显著差异。本研究中健康儿童的氧化应激指标似乎与健康成年人相似，并且抗氧化剂补充并未使其发生改变。饮食记录分析表明，水果和蔬菜的平均摄入量（2.75份/天）与美国儿童全国平均摄入量相似。
应用/结论：本研究提供了关于健康儿童氧化应激主题方面的原始信息。本研究结果可作为参考基线标志物，用于临床饮食评估以及未来针对健康儿童和处于氧化应激水平升高疾病状态儿童的研究。
