Kadotani Naoto, Ikegami Masato
Department of Bioscience, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Pest Manag Sci. 2002 Nov;58(11):1137-42. doi: 10.1002/ps.581.
Patchouli (Pogostemon cablin (Blanco) Benth), an aromatic crop which yields an essential oil, is widely cultivated in South-east Asia. Patchouli mild mosaic virus (PaMMV) infects patchouli plants and causes decrease in leaf biomass and essential oil yield. Transgenic patchouli plants with PaMMV coat protein precursor (CP-P) gene have been produced by Agrobacterium-mediated transformation. PaMMV CP-P gene integration into the patchouli genome was confirmed by the PCR method and by Southern blot analysis. The transformants were estimated to contain one to three copy genes using Southern blot analysis. The transformant with three copy genes was tested for the resistance to PaMMV by artificially inoculating plants grown in an environmentally controlled cabinet, and this transformant was found to be highly resistant to PaMMV. The transgenic patchouli plant with PaMMV CP-P gene should provide valuable material for protecting against PaMMV.
广藿香（Pogostemon cablin (Blanco) Benth）是一种能产出精油的芳香作物，在东南亚广泛种植。广藿香轻度花叶病毒（PaMMV）感染广藿香植株，导致叶片生物量和精油产量下降。通过农杆菌介导转化法已培育出携带PaMMV外壳蛋白前体（CP-P）基因的转基因广藿香植株。采用PCR方法和Southern杂交分析证实了PaMMV CP-P基因已整合到广藿香基因组中。利用Southern杂交分析估计转化体含有1至3个拷贝基因。通过在环境控制箱中种植的植株进行人工接种，对含有3个拷贝基因的转化体进行了抗PaMMV测试，发现该转化体对PaMMV具有高度抗性。携带PaMMV CP-P基因的转基因广藿香植株应为抵御PaMMV提供有价值的材料。
