Ishida Isao, Tukahara Masayoshi, Yoshioka Masaharu, Ogawa Toshiya, Kakitani Mokoto, Toguri Toshihiro
Pharmaceutical Division, Kirin Brewery Co Ltd, Tokyo 104-8288, Japan.
Pest Manag Sci. 2002 Nov;58(11):1132-6. doi: 10.1002/ps.536.
Many pathogenic plant viruses are RNA viruses, which initiate production of double-stranded RNA intermediates when they replicate in host plant cells. Introduction of double-stranded RNA-specific ribonucleases such as the Schizosaccharomyces pombe derived pac I protein and animal cell derived interferon-induced 2',5'-oligoadenylate synthetase (2-5 Aase)/ribonuclease L (RNase L) system into various plants may make plants resistant to various pathogenic viruses and viroids. We have demonstrated that pac I and 2-5 Aase/RNase L transgenic tobacco plants are resistant to various viruses including tobacco mosaic virus, cucumber mosaic virus and potato virus Y. In addition, pac I transgenic potato plants are resistant to potato spindle tuber viroid. Using Agrobacterium-mediated transformation, we have established a transformation system for chrysanthemum plants and have recently developed pac I transgenic chrysanthemum (Dendranthema grandiflora cv Reagan) resistant to chrysanthemum stunt viroid and have grown them in isolated fields for an evaluation of their effects.
许多致病性植物病毒是RNA病毒，当它们在宿主植物细胞中复制时会启动双链RNA中间体的产生。将双链RNA特异性核糖核酸酶，如裂殖酵母来源的Pac I蛋白和动物细胞来源的干扰素诱导的2',5'-寡腺苷酸合成酶（2-5 Aase）/核糖核酸酶L（RNase L）系统导入各种植物中，可能会使植物对各种致病性病毒和类病毒产生抗性。我们已经证明，Pac I和2-5 Aase/RNase L转基因烟草植物对包括烟草花叶病毒、黄瓜花叶病毒和马铃薯Y病毒在内的各种病毒具有抗性。此外，Pac I转基因马铃薯植物对马铃薯纺锤块茎类病毒具有抗性。利用农杆菌介导的转化，我们建立了菊花植物的转化系统，最近还培育出了对菊花矮化类病毒具有抗性的Pac I转基因菊花（菊花品种Reagan），并将它们种植在隔离的田地中以评估其效果。
