Kozlov D G, Surina E R, Efremov B D, Veĭko V P, Benevolenskiĭ S V
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545 Russia.
Genetika. 2002 Oct;38(10):1324-9.
It is known that upon STA2 gene expression in Saccharomyces cerevisiae cells, two transcripts of different lengths are formed. These fragments contain different start codons of translation (AUG1 and AUG2) located in the same reading frame. The ratio between expression levels of proteins translated from AUG1 and AUG2 was invariably about 2:7 and did not depend on the choice of the reporter product (secreted glucoamylase (GA) or beta-galactosidase accumulated in cells). Neither this ratio depended on glucose repression/derepression. Based on the assumed proportional relationship between levels of transcription and translation of the STA2 gene in yeast cells, all these results cumulatively indicate that the two STA2 transcripts are coregulated. The production of secreted GA was also shown to be markedly stimulated at the posttranscriptional level under conditions of glucose repression.
已知在酿酒酵母细胞中STA2基因表达时，会形成两种不同长度的转录本。这些片段包含位于同一阅读框内的不同翻译起始密码子（AUG1和AUG2）。从AUG1和AUG2翻译的蛋白质表达水平之间的比例始终约为2:7，且不依赖于报告产物的选择（分泌型葡糖淀粉酶（GA）或细胞内积累的β-半乳糖苷酶）。该比例也不依赖于葡萄糖阻遏/去阻遏。基于酵母细胞中STA2基因转录水平与翻译水平之间假定的比例关系，所有这些结果累积表明这两种STA2转录本受到共同调控。在葡萄糖阻遏条件下，分泌型GA的产生在转录后水平也被显著刺激。
