Nemtsev S V, Gamzazade A I, Rogozhin S V, Bykova V M, Bykov V P
Bioinzheneriya Center, Russian Academy of Sciences, pr. Shestidesyatiletiya Oktyabrya 7-1, Moscow, 117312 Russia.
Prikl Biokhim Mikrobiol. 2002 Nov-Dec;38(6):609-15.
Chitin isolated enzymatically from Antarctic krill shells was dissolved in aqueous NaOH by freezing and thawing to create homogenous conditions. Deacetylation was performed at room temperature or heating. The degree of deacetylation, molecular weight, and dynamic viscosity of solutions were estimated in chitosan samples. Deacetylation of chitin under homogenous conditions was optimized. Chitosans with molecular weights of 180-220 and 250-300 kDa were obtained from the chitins of Antarctic krill and Northern shrimps, respectively.
通过冷冻和解冻将从南极磷虾壳中酶法分离得到的几丁质溶解于氢氧化钠水溶液中，以创造均匀条件。脱乙酰作用在室温或加热条件下进行。对壳聚糖样品中的脱乙酰度、分子量和溶液的动态粘度进行了估算。优化了几丁质在均匀条件下的脱乙酰作用。分别从南极磷虾和北方虾的几丁质中获得了分子量为180 - 220 kDa和250 - 300 kDa的壳聚糖。
