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一株 Aureobasidium 属菌株中β-葡萄糖苷酶和β-木糖苷酶的产生、表征及性质

Production, characterization and properties of beta-glucosidase and beta-xylosidase from a strain of Aureobasidium sp.

作者信息

Iembo T, Da-Silva R, Pagnocca F C, Gomes E

机构信息

Laboratório de Bioquímica e Microbiologia Aplikada, Institute de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), São José do Rio Preto, SP-Brazil.

出版信息

Prikl Biokhim Mikrobiol. 2002 Nov-Dec;38(6):639-43.

Abstract

beta-glucosidase and beta-xylosidase production by a yeast-like Aureobasidium sp. was carried out during solid-state and submerged fermentation using different carbon sources and crude enzymes were characterized. beta-glucosidase and beta-xylosidase gave optimum activity at pH 2.0-2.5 and 3.0, respectively. These enzymes presented maximal activity at 65 degrees, and were stable in a wide pH range and high temperatures.

摘要

一株类酵母金色担子菌(Aureobasidium sp.)在固态和深层发酵过程中利用不同碳源生产β-葡萄糖苷酶和β-木糖苷酶,并对粗酶进行了表征。β-葡萄糖苷酶和β-木糖苷酶分别在pH 2.0 - 2.5和3.0时具有最佳活性。这些酶在65摄氏度时呈现最大活性,并且在较宽的pH范围和高温下都很稳定。

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