Pericin D, Jarak M
Faculty of Technology, University of Novi Sad, Yugoslavia.
Acta Microbiol Immunol Hung. 1995;42(1):29-37.
Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al. is an important phytopathogenic fungus which causes stem canker of sunflower. When grown in submerged cultures in the presence of milled wheat chaff as a carbon source this phytopathogen produced extracellular and intracellular beta-glucosidases. The optimum pH of these enzymes was 4.8. The temperature of crude intracellular beta-glucosidase activity was at 60 degrees C, whereas the optimum activity of crude extracellular beta-glucosidase was observed in a wide range of temperature between 40 and 70 degrees C. Although, extracellular and intracellular beta-glucosidase activities had identical pH and similar temperature optima, the thermal stability of the intracellular enzyme was significantly higher.
向日葵拟茎点霉（Diaporthe (Phomopsis) helianthi Munt.-Cvet. 等人）是一种重要的植物病原真菌，可引起向日葵茎溃疡病。当这种植物病原菌在以磨碎的麦麸作为碳源的液体培养物中生长时，会产生细胞外和细胞内β-葡萄糖苷酶。这些酶的最适pH值为4.8。粗制细胞内β-葡萄糖苷酶活性的温度为60℃，而粗制细胞外β-葡萄糖苷酶的最适活性在40至70℃的较宽温度范围内观察到。虽然细胞外和细胞内β-葡萄糖苷酶活性具有相同的pH值和相似的最适温度，但细胞内酶的热稳定性明显更高。
