Cossette Louis J, Gaumond Isabelle, Martinoli Maria-Grazia
Department of Biochemistry, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada.
Endocrine. 2002 Aug;18(3):303-8. doi: 10.1385/ENDO:18:3:303.
It is now well recognized that estrogenic signaling mechanisms are far more complex than once thought. Several crosstalks between the estrogen receptor and other signaling pathways may influence the estrogenic stimulation of cell growth. Thus, the estrogenic effects of several environmental contaminants, now suspected to act as endocrine disrupters, may be influenced by a simultaneous stimulation of other signaling pathways. The aim of this study was to investigate whether the growth response of two estrogen-responsive cell lines, MCF-7 and GH3, treated with xenoestrogens might be affected by the addition of growth factors to their culture medium. Cells were treated with two known xenoestrogens, endosulfan and chlordane, alone or in the presence of insulin-like growth factor-1 and epidermal growth factor, respectively, and their growth was measured using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxyanilide proliferation assay. Our results show that treatment with endosulfan or chlordane as well as treatment with growth factors increased cell growth, while the administration of xenoestrogens together with growth factors triggered a partly additive response with no antagonist or synergistic effect. These results sustain a role for xenoestrogens in cellular growth.
现在人们已经充分认识到，雌激素信号传导机制远比曾经认为的要复杂得多。雌激素受体与其他信号通路之间的几种相互作用可能会影响雌激素对细胞生长的刺激。因此，现在怀疑作为内分泌干扰物的几种环境污染物的雌激素效应，可能会受到其他信号通路同时刺激的影响。本研究的目的是调查用异种雌激素处理的两种雌激素反应性细胞系MCF-7和GH3的生长反应，是否会因在其培养基中添加生长因子而受到影响。细胞分别用两种已知的异种雌激素硫丹和氯丹单独处理，或在胰岛素样生长因子-1和表皮生长因子存在的情况下处理，并使用2,3-双(2-甲氧基-4-硝基-5-磺基苯基)-2H-四唑-5-羧基苯胺增殖试验测量它们的生长。我们的结果表明，用硫丹或氯丹处理以及用生长因子处理都会增加细胞生长，而将异种雌激素与生长因子一起施用会引发部分相加反应，没有拮抗或协同作用。这些结果支持了异种雌激素在细胞生长中的作用。
