采用梯度反相液相色谱-紫外吸收检测法同时测定人血浆中的塞来昔布、羟基塞来昔布和羧基塞来昔布。
Simultaneous determination of celecoxib, hydroxycelecoxib, and carboxycelecoxib in human plasma using gradient reversed-phase liquid chromatography with ultraviolet absorbance detection.
作者信息
Störmer Elke, Bauer Steffen, Kirchheiner Julia, Brockmöller Jürgen, Roots Ivar
机构信息
Institute of Clinical Pharmacology, University Medical Center Charité, Humboldt University Berlin, Schumannstrasse 20/21, 10098, Berlin, Germany.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jan 5;783(1):207-12. doi: 10.1016/s1570-0232(02)00658-x.
A new HPLC method for the simultaneous determination of celecoxib, carboxycelecoxib and hydroxycelecoxib in human plasma samples has been developed. Following a solid-phase extraction procedure, the samples were separated by gradient reversed-phase HLPC (C(18)) and quantified using UV detection at 254 nm. The method was linear over the concentration range 10-500 ng/ml. The intra-assay variability for the three analytes ranged from 4.0 to 12.6% and the inter-assay variability from 4.9 to 14.2%. The achieved limits of quantitation (LOQ) of 10 ng/ml for each analyte allowed the determination of the pharmacokinetic parameters of the analytes after administration of 100 mg celecoxib.
已开发出一种用于同时测定人血浆样品中塞来昔布、羧基塞来昔布和羟基塞来昔布的高效液相色谱新方法。经过固相萃取程序后，样品通过梯度反相高效液相色谱（C(18)）进行分离，并使用254 nm的紫外检测进行定量。该方法在10 - 500 ng/ml的浓度范围内呈线性。三种分析物的批内变异系数范围为4.0%至12.6%，批间变异系数范围为4.9%至14.2%。每种分析物10 ng/ml的定量限使得在给予100 mg塞来昔布后能够测定分析物的药代动力学参数。
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