采用高效液相色谱法测定猫血浆样本中的德拉考昔。
Determination of deracoxib in feline plasma samples using high performance liquid chromatography.
作者信息
Cox Sherry K, Roark Jacob, Gassel Adam, Tobias Karen
机构信息
Department of Comparative Medicine, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37966, USA.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 May 5;819(1):181-4. doi: 10.1016/j.jchromb.2005.01.025.
A new HPLC procedure for the determination of deracoxib, a selective cyclooxygenase-2 inhibitor, has been developed and validated. Following a liquid-liquid extraction using isopropyl alcohol and chloroform, samples were separated by isocratic reversed-phase HPLC on an Atlantis C18 column and quantified using UV detection at 252 nm. The mobile phase was a mixture of 10 mM potassium phosphate (pH 4.5) and acetonitrile, with a flow-rate of 1.0 ml/min. The procedure produced a linear curve over the concentration range 10-1500 ng/ml. The development of the assay allowed the determination of pharmacokinetic parameters after oral administration of deracoxib in cats and would be suitable for other pharmacokinetic studies.
已开发并验证了一种用于测定选择性环氧化酶-2抑制剂德拉考昔的新高效液相色谱法。使用异丙醇和氯仿进行液-液萃取后，样品在Atlantis C18柱上通过等度反相高效液相色谱法分离，并在252 nm处使用紫外检测进行定量。流动相为10 mM磷酸钾（pH 4.5）和乙腈的混合物，流速为1.0 ml/min。该方法在10-1500 ng/ml的浓度范围内产生线性曲线。该分析方法的开发使得能够测定猫口服德拉考昔后的药代动力学参数，并且适用于其他药代动力学研究。
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