Seger Yvette R, García-Cao Marta, Piccinin Sara, Cunsolo Crocifissa Lo, Doglioni Claudio, Blasco María A, Hannon Gregory J, Maestro Roberta
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724, USA.
Cancer Cell. 2002 Nov;2(5):401-13. doi: 10.1016/s1535-6108(02)00183-6.
Our knowledge of the transformation process has emerged largely from studies of primary rodent cells and animal models. However, numerous attempts to transform human cells using oncogene combinations that are effective in rodents have proven unsuccessful. These findings strongly argue for the study of homologous experimental systems. Here we report that the combined expression of adenovirus E1A, Ha-RasV12, and MDM2 is sufficient to convert a normal human cell into a cancer cell. Notably, transformation did not require telomerase activation. Therefore, we provide evidence that activation of telomere maintenance strategies is not an obligate characteristic of tumorigenic human cells.
我们对转化过程的认识很大程度上源于对原代啮齿动物细胞和动物模型的研究。然而，使用在啮齿动物中有效的癌基因组合来转化人类细胞的众多尝试均以失败告终。这些发现有力地支持了对同源实验系统的研究。在此，我们报告腺病毒E1A、Ha-RasV12和MDM2的联合表达足以将正常人类细胞转化为癌细胞。值得注意的是，转化并不需要端粒酶激活。因此，我们提供了证据表明端粒维持策略的激活并非致瘤性人类细胞的必然特征。
