Liu Yan, Wu Huiqin, Ge Fahuan
Guangdong College of Pharmacy, Guangzhou 510224.
Zhong Yao Cai. 2002 Sep;25(9):635-7.
To study the relationship between anticonvulsive effect of three extracts from Radix Bupleuri and their chemical compositions.
Volatile oil component, saikosaponin component and water-soluble component were extracted with SFE-CO2 from Radix Bupleuri, their effect on MES and PTZ seizure models had been studied, and their compositions were analyzed by GC-MS and HPLC.
Compared with the control, volatile oil component and saikosaponin component significantly decreased anticonvulsive occurrence on MES, the main chemical compositions of them was linoleic acid; water-soluble component significantly decreased anticonvulsive occurrence on PTZ seizure, but most of its compositions were glycerol and xylitol.
Linoleic acid from Radix Bupleuri maybe fight against MES seizure, while glycerol and xylitol could fight against PTZ seizure.
研究柴胡三种提取物的抗惊厥作用与其化学成分之间的关系。
采用超临界流体萃取法（SFE-CO₂）从柴胡中提取挥发油成分、柴胡皂苷成分和水溶性成分，研究其对最大电休克（MES）和戊四氮（PTZ）惊厥模型的作用，并通过气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）分析其成分。
与对照组相比，挥发油成分和柴胡皂苷成分显著降低MES惊厥的发生率，其主要化学成分是亚油酸；水溶性成分显著降低PTZ惊厥的发生率，但其大部分成分是甘油和木糖醇。
柴胡中的亚油酸可能对抗MES惊厥，而甘油和木糖醇可对抗PTZ惊厥。
