采用反相高效液相色谱-脉冲安培检测法分析柴胡及柴胡疏肝散中的柴胡皂苷。
Analysis of saikosaponins in Bupleuri Radix and Caihu-shugan-san using reversed-phase HPLC with pulsed amperometric detection.
机构信息
Department of Preventive and Social Dentistry, Graduate School, Kyung Hee University, Seoul, South Korea.
出版信息
J Sep Sci. 2011 Mar;34(6):651-8. doi: 10.1002/jssc.201000784. Epub 2011 Feb 11.
A simple and sensitive reversed-phase (RP) HPLC coupled with pulsed amperometric detection (PAD) method was developed to determine the saikosaponin content in Bupleuri Radix or Caihu-shugan-san. Four saikosaponins in Bupleuri Radix and Caihu-shugan-san were extracted with a 6:4 solution of 10 mM sodium phosphate buffer (pH 8)/100% ethanol. Pulsed amperometric detection of carbohydrates in four major saikosaponins was highly sensitive when used with a water-acetonitrile gradient on an alkaline RP column with a post-column delivery system. The limits of detection (S/N=3) and of quantification (S/N=10) of saikosaponins were 0.01-0.02 and 0.03-0.05 μg/mL, respectively. The intra- and inter-day precision (RSDs) were each <9.7% and the average recoveries were 95.0-97.6% in Bupleuri Radix. This method can be used to analyze saikosaponins in Bupleuri Radix and Caihu-shugan-san.
建立了一种简单灵敏的反相高效液相色谱法（RP-HPLC）结合脉冲安培检测（PAD）方法，用于测定柴胡或柴胡疏肝散中柴胡皂苷的含量。采用 6：4 的 10 mM 磷酸钠缓冲液（pH 8）/100%乙醇溶液从柴胡和柴胡疏肝散中提取 4 种柴胡皂苷。在碱性 RP 柱上采用水-乙腈梯度洗脱和柱后输送系统，对 4 种主要柴胡皂苷中的碳水化合物进行脉冲安培检测，具有很高的灵敏度。柴胡皂苷的检测限（S/N=3）和定量限（S/N=10）分别为 0.01-0.02 和 0.03-0.05 μg/mL。在柴胡中，日内和日间精密度（RSD）均<9.7%，平均回收率为 95.0-97.6%。该方法可用于分析柴胡和柴胡疏肝散中的柴胡皂苷。
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