Feng Gong-kan, Xie Bing-fen, Liu Zong-chao, Zhu Xiao-feng, Ye Yan-li
Cancer Institute, Cancer Center, Sun Yet-sen University, Guangzhou 510060, P. R. China.
Ai Zheng. 2002 Apr;21(4):392-4.
It was reported that tea polyphenol(TP) possess preventive and anticancer effects on various human cancers. However the report about TP against human nasopharyngeal carcinoma(NPC) was very rare. Our previous studies have also suggested that TP has antiproliferative effect and may induce apoptosis in human NPC cell. In order to further explore the antitumor effect, the authors investigated the growth inhibition effect of TP on various human NPC cell lines and xenograft tumors of human NPC in nude mice.
Antiproliferative effect of TP against seven human NPC cell lines was tested by MTT method. Antitumor effect of TP was determined using the xenografts models of human NPC cell (CNE2) in nude mice.
The fifty percent inhibition concentration (IC50) of TP against NPC cell lines (NPC/HK1, CNE1, CNE2, HNE1, HNE2, SUNE1, and Fadu cells) were calculated to be 55.83, 98.43, 119.21, 127.74, 158.07, 160.72, and 163.59 micrograms/ml, respectively. Average inhibitory rates of TP against xenograft tumors of human NPC in nude mice were 12.7% (P > 0.05), under the dosage of 5 mg.(kg..d)-1, ig x 18 d. Under the dosages of 10 mg.(kg..d)-1, ig x 18 d and 20 mg.(kg..d)-1, ig x 18 average inhibitory rates were 31.0% and 38.5%, (P < 0.01), respectively.
The results indicated that TP possessed different antiproliferative effect on seven human NPC cell lines in vitro and on xenograft tumor of human NPC in nude mice.
据报道，茶多酚（TP）对多种人类癌症具有预防和抗癌作用。然而，关于TP对人类鼻咽癌（NPC）作用的报道非常罕见。我们之前的研究也表明，TP具有抗增殖作用，并可能诱导人NPC细胞凋亡。为了进一步探讨其抗肿瘤作用，作者研究了TP对多种人NPC细胞系以及裸鼠人NPC移植瘤的生长抑制作用。
采用MTT法检测TP对七种人NPC细胞系的抗增殖作用。利用人NPC细胞（CNE2）在裸鼠中的移植瘤模型确定TP的抗肿瘤作用。
TP对NPC细胞系（NPC/HK1、CNE1、CNE2、HNE1、HNE2、SUNE1和Fadu细胞）的半数抑制浓度（IC50）分别计算为55.83、98.43、119.21、127.74、158.07、160.72和163.59微克/毫升。在5毫克/（千克·天）-1的剂量下，灌胃18天，TP对裸鼠人NPC移植瘤的平均抑制率为12.7%（P>0.05）。在10毫克/（千克·天）-1的剂量下，灌胃18天，平均抑制率为31.0%；在20毫克/（千克·天）-1的剂量下，灌胃18天，平均抑制率为38.5%，（P<0.01）。
结果表明，TP对七种人NPC细胞系体外培养以及裸鼠人NPC移植瘤具有不同的抗增殖作用。
